BMRB Entry 50221
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50221
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Staphylococcus aureus EF-GC3 methyl side chain chemical shifts PubMed: 32999060
Deposition date: 2020-04-01 Original release date: 2020-10-07
Authors: Tomlinson, Jennifer; Kalverda, Arnout
Citation: Tomlinson, Jennifer; Kalverda, Arnout; Calabrese, Antonio. "Fusidic acid resistance through changes in the dynamics of the drug target" Proc. Natl. Acad. Sci. U. S. A. 117, 25523-25531 (2020).
Assembly members:
entity_1, polymer, 301 residues, 33424.6284 Da.
Natural source: Common Name: Staphylococcus aureus Taxonomy ID: 1280 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus aureus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-29b
Entity Sequences (FASTA):
entity_1: MEFPEPVIHLSVEPKSKADQ
DKMTQALVKLQEEDPTFHAH
TDEETGQVIIGGMGELHLDI
LVDRMKKEFNVECNVGAPMV
SYRETFKSSAQVQGKFSRQS
GGRGQYGDVHIEFTPNETGA
GFEFENAIVGGVVPREYIPS
VEAGLKDAMENGVLAGYPLI
DVKAKLYDGSYHDVDSSEMA
FKIAASLALKEAAKKCDPVI
LEPMMKVTIEMPEEYMGDIM
GDVTSRRGRVDGMEPRGNAQ
VVNAYVPLSEMFGYATSLRS
NTQGRGTYTMYFDHYAEVPK
SIAEDIIKKNKGELEHHHHH
H
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 265 |
| 1H chemical shifts | 361 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EF-GC3 | 1 |
Entities:
Entity 1, EF-GC3 301 residues - 33424.6284 Da.
This truncated protein begins at residue 401 of the full length EF-G protein and comprises domains III-V of EF-G. Residues 694-701 represent a non-native affinity tag
| 1 | MET | GLU | PHE | PRO | GLU | PRO | VAL | ILE | HIS | LEU | ||||
| 2 | SER | VAL | GLU | PRO | LYS | SER | LYS | ALA | ASP | GLN | ||||
| 3 | ASP | LYS | MET | THR | GLN | ALA | LEU | VAL | LYS | LEU | ||||
| 4 | GLN | GLU | GLU | ASP | PRO | THR | PHE | HIS | ALA | HIS | ||||
| 5 | THR | ASP | GLU | GLU | THR | GLY | GLN | VAL | ILE | ILE | ||||
| 6 | GLY | GLY | MET | GLY | GLU | LEU | HIS | LEU | ASP | ILE | ||||
| 7 | LEU | VAL | ASP | ARG | MET | LYS | LYS | GLU | PHE | ASN | ||||
| 8 | VAL | GLU | CYS | ASN | VAL | GLY | ALA | PRO | MET | VAL | ||||
| 9 | SER | TYR | ARG | GLU | THR | PHE | LYS | SER | SER | ALA | ||||
| 10 | GLN | VAL | GLN | GLY | LYS | PHE | SER | ARG | GLN | SER | ||||
| 11 | GLY | GLY | ARG | GLY | GLN | TYR | GLY | ASP | VAL | HIS | ||||
| 12 | ILE | GLU | PHE | THR | PRO | ASN | GLU | THR | GLY | ALA | ||||
| 13 | GLY | PHE | GLU | PHE | GLU | ASN | ALA | ILE | VAL | GLY | ||||
| 14 | GLY | VAL | VAL | PRO | ARG | GLU | TYR | ILE | PRO | SER | ||||
| 15 | VAL | GLU | ALA | GLY | LEU | LYS | ASP | ALA | MET | GLU | ||||
| 16 | ASN | GLY | VAL | LEU | ALA | GLY | TYR | PRO | LEU | ILE | ||||
| 17 | ASP | VAL | LYS | ALA | LYS | LEU | TYR | ASP | GLY | SER | ||||
| 18 | TYR | HIS | ASP | VAL | ASP | SER | SER | GLU | MET | ALA | ||||
| 19 | PHE | LYS | ILE | ALA | ALA | SER | LEU | ALA | LEU | LYS | ||||
| 20 | GLU | ALA | ALA | LYS | LYS | CYS | ASP | PRO | VAL | ILE | ||||
| 21 | LEU | GLU | PRO | MET | MET | LYS | VAL | THR | ILE | GLU | ||||
| 22 | MET | PRO | GLU | GLU | TYR | MET | GLY | ASP | ILE | MET | ||||
| 23 | GLY | ASP | VAL | THR | SER | ARG | ARG | GLY | ARG | VAL | ||||
| 24 | ASP | GLY | MET | GLU | PRO | ARG | GLY | ASN | ALA | GLN | ||||
| 25 | VAL | VAL | ASN | ALA | TYR | VAL | PRO | LEU | SER | GLU | ||||
| 26 | MET | PHE | GLY | TYR | ALA | THR | SER | LEU | ARG | SER | ||||
| 27 | ASN | THR | GLN | GLY | ARG | GLY | THR | TYR | THR | MET | ||||
| 28 | TYR | PHE | ASP | HIS | TYR | ALA | GLU | VAL | PRO | LYS | ||||
| 29 | SER | ILE | ALA | GLU | ASP | ILE | ILE | LYS | LYS | ASN | ||||
| 30 | LYS | GLY | GLU | LEU | GLU | HIS | HIS | HIS | HIS | HIS | ||||
| 31 | HIS |
Samples:
sample_1: EF-GC3, [U-100% 13C; U-100% 15N; U-80% 2H], 0.25 mM; TrisHCl 20 mM; NaCl 300 mM; DTT 1 mM
sample_conditions_1: ionic strength: 0.310 M; pH: 8.000; pressure: 1.000 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr_Analysis v2.1 - assignment
NMRPipe - processing
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker Avance 750 MHz
Related Database Links:
| NCBI | P68790 |