BMRB Entry 50228

Name: 1H, 13C and 15N resonance assignments for the microtubule binding region of the kinetoplastid kinetochore protein KKT4 145-232
Published: 2020-07-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50228

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignments for the microtubule binding region of the kinetoplastid kinetochore protein KKT4 145-232

Deposition date:

2020-04-09

Original release date:

2020-07-24

Authors:

Ludzia, Patryk; Akiyoshi, Bungo; Redfield, Christina

Citation:

Citation: Ludzia, Patryk; Akiyoshi, Bungo; Redfield, Christina. "1 H, 13 C and 15 N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei" Biomol. NMR Assignments 14, 309-315 (2020).
PubMed: 32696260

Assembly members:

Assembly members:
entity_1, polymer, 90 residues, 10335.71 Da.

Natural source:

Natural source: Common Name: Trypanosoma brucei brucei Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSF_Duet-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMRSEPVVDTQRVLDLEEEV ARLKRTIGHLQGVVEEKESA LEKHATQHNLEVHEMKKNYE LKIKSLTQTHEAAVRKLVSA QELVTAARNY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	249
15N chemical shifts	92
1H chemical shifts	100

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KKT4 145-232	1

Entities:

Entity 1, KKT4 145-232 90 residues - 10335.71 Da.

Residues 1-2 (143S, 144M) represent part of a remained linker after TEV protease cleavage.

1	SER	MET	ARG	SER	GLU	PRO	VAL	VAL	ASP	THR
2	GLN	ARG	VAL	LEU	ASP	LEU	GLU	GLU	GLU	VAL
3	ALA	ARG	LEU	LYS	ARG	THR	ILE	GLY	HIS	LEU
4	GLN	GLY	VAL	VAL	GLU	GLU	LYS	GLU	SER	ALA
5	LEU	GLU	LYS	HIS	ALA	THR	GLN	HIS	ASN	LEU
6	GLU	VAL	HIS	GLU	MET	LYS	LYS	ASN	TYR	GLU
7	LEU	LYS	ILE	LYS	SER	LEU	THR	GLN	THR	HIS
8	GLU	ALA	ALA	VAL	ARG	LYS	LEU	VAL	SER	ALA
9	GLN	GLU	LEU	VAL	THR	ALA	ALA	ARG	ASN	TYR

Name	Sample	Sample state	Sample conditions
2D BEST TROSY	sample_2	isotropic	sample_conditions_1
2D BEST TROSY	sample_1	isotropic	sample_conditions_1
2D BEST TROSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D BT HNCA	sample_1	isotropic	sample_conditions_1
3D BT HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D BT HNCACB	sample_1	isotropic	sample_conditions_1
3D BT HNCO	sample_1	isotropic	sample_conditions_1
3D BT HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1

UniProt	A0A3L6L4L8
AlphaFold	A0A3L6L4L8

BMRB Entry 50228

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	SER	MET	ARG	SER	GLU	PRO	VAL	VAL	ASP	THR
2	GLN	ARG	VAL	LEU	ASP	LEU	GLU	GLU	GLU	VAL
3	ALA	ARG	LEU	LYS	ARG	THR	ILE	GLY	HIS	LEU
4	GLN	GLY	VAL	VAL	GLU	GLU	LYS	GLU	SER	ALA
5	LEU	GLU	LYS	HIS	ALA	THR	GLN	HIS	ASN	LEU
6	GLU	VAL	HIS	GLU	MET	LYS	LYS	ASN	TYR	GLU
7	LEU	LYS	ILE	LYS	SER	LEU	THR	GLN	THR	HIS
8	GLU	ALA	ALA	VAL	ARG	LYS	LEU	VAL	SER	ALA
9	GLN	GLU	LEU	VAL	THR	ALA	ALA	ARG	ASN	TYR

1	SER	MET	ARG	SER	GLU	PRO	VAL	VAL	ASP	THR
2	GLN	ARG	VAL	LEU	ASP	LEU	GLU	GLU	GLU	VAL
3	ALA	ARG	LEU	LYS	ARG	THR	ILE	GLY	HIS	LEU
4	GLN	GLY	VAL	VAL	GLU	GLU	LYS	GLU	SER	ALA
5	LEU	GLU	LYS	HIS	ALA	THR	GLN	HIS	ASN	LEU
6	GLU	VAL	HIS	GLU	MET	LYS	LYS	ASN	TYR	GLU
7	LEU	LYS	ILE	LYS	SER	LEU	THR	GLN	THR	HIS
8	GLU	ALA	ALA	VAL	ARG	LYS	LEU	VAL	SER	ALA
9	GLN	GLU	LEU	VAL	THR	ALA	ALA	ARG	ASN	TYR

1	SER	MET	ARG	SER	GLU	PRO	VAL	VAL	ASP	THR
2	GLN	ARG	VAL	LEU	ASP	LEU	GLU	GLU	GLU	VAL
3	ALA	ARG	LEU	LYS	ARG	THR	ILE	GLY	HIS	LEU
4	GLN	GLY	VAL	VAL	GLU	GLU	LYS	GLU	SER	ALA
5	LEU	GLU	LYS	HIS	ALA	THR	GLN	HIS	ASN	LEU
6	GLU	VAL	HIS	GLU	MET	LYS	LYS	ASN	TYR	GLU
7	LEU	LYS	ILE	LYS	SER	LEU	THR	GLN	THR	HIS
8	GLU	ALA	ALA	VAL	ARG	LYS	LEU	VAL	SER	ALA
9	GLN	GLU	LEU	VAL	THR	ALA	ALA	ARG	ASN	TYR