BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50233

Title: Model-free analysis of 15N spin-relaxation data for TRBP2-dsRBD1   PubMed: 35134335

Deposition date: 2020-04-14 Original release date: 2022-02-16

Authors: Paithankar, Harshad; Chugh, Jeetender

Citation: Paithankar, Harshad; Tarang, Guneet Singh; Parvez, Firdousi; Marathe, Aniket; Joshi, Manali; Chugh, Jeetender. "Relay of intrinsic microsecond timescale conformational dynamics observed in an RNA-binding domain of TRBP upon enthalpy-driven binding of dsRNA"  Biophys. J. ., .-. (2022).

Assembly members:
entity_1, polymer, 108 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSV272

Entity Sequences (FASTA):
entity_1: GGAMSEEEQGSGTTTGCGLP SIEQMLAANPGKTPISLLQE YGTRIGKTPVYDLLKAEGQA HQPNFTFRVTVGDTSCTGQG PSKKAAKHKAAEVALKHLKG GSMLEPAL

Data typeCount
T1 relaxation values138
T2 relaxation values138
heteronuclear NOE values138

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TRBP2-dsRBD11

Entities:

Entity 1, TRBP2-dsRBD1 108 residues - Formula weight is not available

N-terminal residues GGA are non-native to the protein and are left after the cleavage of the fusion tag using protease.

1   GLYGLYALAMETSERGLUGLUGLUGLNGLY
2   SERGLYTHRTHRTHRGLYCYSGLYLEUPRO
3   SERILEGLUGLNMETLEUALAALAASNPRO
4   GLYLYSTHRPROILESERLEULEUGLNGLU
5   TYRGLYTHRARGILEGLYLYSTHRPROVAL
6   TYRASPLEULEULYSALAGLUGLYGLNALA
7   HISGLNPROASNPHETHRPHEARGVALTHR
8   VALGLYASPTHRSERCYSTHRGLYGLNGLY
9   PROSERLYSLYSALAALALYSHISLYSALA
10   ALAGLUVALALALEULYSHISLEULYSGLY
11   GLYSERMETLEUGLUPROALALEU

Samples:

sample_1: TRBP2-dsRBD1, [U-100% 15N], 1.8 mM; D2O, [U-2H], 10%; DTT 1 mM; sodium phosphate 10 mM; sodium chloride 100 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
T1/R1 relaxationsample_1isotropicsample_conditions_1
T2/R2 relaxationsample_1isotropicsample_conditions_1
1H-15N heteronoesample_1isotropicsample_conditions_1
T1/R1 relaxationsample_1isotropicsample_conditions_1
T2/R2 relaxationsample_1isotropicsample_conditions_1
1H-15N heteronoesample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5 - collection

NMRPipe - processing

NMRFAM-SPARKY - peak picking

Relax v4.0.3 - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 750 MHz