BMRB Entry 50236
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PDB ID: 6wlh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50236
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Nishikawa, Tadateru; Wojciak, Jonathan; Dyson, Helen Jane; Wright, Peter. "RNA Binding by the KTS Splice Variants of Wilms' Tumor Suppressor Protein WT1" Biochemistry 59, 3889-3901 (2020).
PubMed: 32955251
Assembly members:
entity_1, polymer, 117 residues, Formula weight is not available
entity_2, polymer, 29 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pWT1-4-
Entity Sequences (FASTA):
entity_1: EKRPFMCAYPGCNKRYFKLS
HLQMHSRKHTGEKPYQCDFK
DCERRFSRSDQLKRHQRRHT
GVKPFQCKTCQRKFSRSDHL
KTHTRTHTGEKPFSCRWPSC
QKKFARSDELVRHHNMH
entity_2: GGGCCACCAACGACAUUGAU
AUGGUGCCC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 473 |
| 15N chemical shifts | 109 |
| 1H chemical shifts | 264 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | protein | 1 |
| 2 | RNA | 2 |
| 3 | ZN, 1 | 3 |
| 4 | ZN, 2 | 3 |
| 5 | ZN, 3 | 3 |
Entities:
Entity 1, protein 117 residues - Formula weight is not available
| 1 | GLU | LYS | ARG | PRO | PHE | MET | CYS | ALA | TYR | PRO | ||||
| 2 | GLY | CYS | ASN | LYS | ARG | TYR | PHE | LYS | LEU | SER | ||||
| 3 | HIS | LEU | GLN | MET | HIS | SER | ARG | LYS | HIS | THR | ||||
| 4 | GLY | GLU | LYS | PRO | TYR | GLN | CYS | ASP | PHE | LYS | ||||
| 5 | ASP | CYS | GLU | ARG | ARG | PHE | SER | ARG | SER | ASP | ||||
| 6 | GLN | LEU | LYS | ARG | HIS | GLN | ARG | ARG | HIS | THR | ||||
| 7 | GLY | VAL | LYS | PRO | PHE | GLN | CYS | LYS | THR | CYS | ||||
| 8 | GLN | ARG | LYS | PHE | SER | ARG | SER | ASP | HIS | LEU | ||||
| 9 | LYS | THR | HIS | THR | ARG | THR | HIS | THR | GLY | GLU | ||||
| 10 | LYS | PRO | PHE | SER | CYS | ARG | TRP | PRO | SER | CYS | ||||
| 11 | GLN | LYS | LYS | PHE | ALA | ARG | SER | ASP | GLU | LEU | ||||
| 12 | VAL | ARG | HIS | HIS | ASN | MET | HIS |
Entity 2, RNA 29 residues - Formula weight is not available
| 1 | G | G | G | C | C | A | C | C | A | A | ||||
| 2 | C | G | A | C | A | U | U | G | A | U | ||||
| 3 | A | U | G | G | U | G | C | C | C |
Entity 3, ZN, 1 - Zn - 65.409 Da.
| 1 | ZN |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks