BMRB Entry 50259
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50259
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Title: 1H, 13C and 15N Resonance Assignments of the Transactivation domain of the Human Forkhead Transcription Factor FOXO4 PubMed: 33450250
Deposition date: 2020-05-04 Original release date: 2021-08-17
Authors: Park, Chin-Ju; Ahn, Dabin; Kim, Jinwoo
Citation: Kim, Jinwoo; Ahn, Dabin; Park, Chin-Ju. "FOXO4 Transactivation Domain Interaction with Forkhead DNA Binding Domain and Effect on Selective DNA Recognition for Transcription Initiation" J. Mol. Biol. 433, 166808-166808 (2021).
Assembly members:
entity_1, polymer, 37 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET His6 GST TEV LIC cloning vector (2G-T)
Entity Sequences (FASTA):
entity_1: DLDLDMYMENLECDMDNIIS
DLMDEGEGLDFNFEPDP
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 100 |
15N chemical shifts | 34 |
1H chemical shifts | 34 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | FOXO4 TAD | 1 |
Entities:
Entity 1, FOXO4 TAD 37 residues - Formula weight is not available
1 | ASP | LEU | ASP | LEU | ASP | MET | TYR | MET | GLU | ASN | ||||
2 | LEU | GLU | CYS | ASP | MET | ASP | ASN | ILE | ILE | SER | ||||
3 | ASP | LEU | MET | ASP | GLU | GLY | GLU | GLY | LEU | ASP | ||||
4 | PHE | ASN | PHE | GLU | PRO | ASP | PRO |
Samples:
sample_1: sodium phosphate 20 mM; sodium chloride 50 mM; DTT 2 mM; FOXO4 TAD, [U-100% 13C; U-100% 15N], 800 uM
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker Avance 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts