BMRB Entry 50270

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50270

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Cre Recombinase Catalytic Domain
Deposition date:
2020-05-12
Original release date:
2021-08-11
Authors:
Unnikrishnan, Aparna; Foster, Mark
Citation:

Citation: Unnikrishnan, Aparna; Amero, Carlos; Yadav, Deepak Kumar; Stachowski, Kye; Potter, Devante; Foster, Mark. "DNA binding induces a cis-to- trans switch in Cre recombinase to enable intasome assembly"  Proc. Natl. Acad. Sci. U. S. A. 117, 24849-24858 (2020).
PubMed: 32968014

Assembly members:

Assembly members:
entity_1, polymer, 217 residues, 23814.1 Da.

Natural source:

Natural source:   Common Name: Escherichia virus P1   Taxonomy ID: 10678   Superkingdom: Viruses   Kingdom: not available   Genus/species: Punavirus Bacteriophage P1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AGERAKQALAFERTDFDQVR SLMENSDRCQDIRNLAFLGI AYNTLLRIAEIARIRVKDIS RTDGGRMLIHIGRTKTLVST AGVEKALSLGVTKLVERWIS VSGVADDPNNYLFCRVRKNG VAAPSATSQLSTRALEGIFE ATHRLIYGAKDDSGQRYLAW SGHSARVGAARDMARAGVSI PEIMQAGGWTNVNIVMNYIR NLDSETGAMVRLLEDGD

Data sets:
Data typeCount
13C chemical shifts595
15N chemical shifts196
1H chemical shifts362

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cre Recombinase Catalytic DomainCre Recombinase Catalytic Domain1

Entities:

Entity 1, Cre Recombinase Catalytic DomainCre Recombinase Catalytic Domain 217 residues - 23814.1 Da.

Residue numbers 127 to 343 in Cre Recombinase

1   ALAGLYGLUARGALALYSGLNALALEUALA
2   PHEGLUARGTHRASPPHEASPGLNVALARG
3   SERLEUMETGLUASNSERASPARGCYSGLN
4   ASPILEARGASNLEUALAPHELEUGLYILE
5   ALATYRASNTHRLEULEUARGILEALAGLU
6   ILEALAARGILEARGVALLYSASPILESER
7   ARGTHRASPGLYGLYARGMETLEUILEHIS
8   ILEGLYARGTHRLYSTHRLEUVALSERTHR
9   ALAGLYVALGLULYSALALEUSERLEUGLY
10   VALTHRLYSLEUVALGLUARGTRPILESER
11   VALSERGLYVALALAASPASPPROASNASN
12   TYRLEUPHECYSARGVALARGLYSASNGLY
13   VALALAALAPROSERALATHRSERGLNLEU
14   SERTHRARGALALEUGLUGLYILEPHEGLU
15   ALATHRHISARGLEUILETYRGLYALALYS
16   ASPASPSERGLYGLNARGTYRLEUALATRP
17   SERGLYHISSERALAARGVALGLYALAALA
18   ARGASPMETALAARGALAGLYVALSERILE
19   PROGLUILEMETGLNALAGLYGLYTRPTHR
20   ASNVALASNILEVALMETASNTYRILEARG
21   ASNLEUASPSERGLUTHRGLYALAMETVAL
22   ARGLEULEUGLUASPGLYASP

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks