BMRB Entry 50274

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50274

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Title:
Orb2A Prion Like Domain
Deposition date:
2020-05-15
Original release date:
2020-10-21
Authors:
Laurents, Douglas; Oroz, Javier
Citation:

Citation: Oroz, Javier; Sousa Felix, Sara; Cabrito, Eurico; Laurents, Douglas. "pH Changes Trigger Structural Transitions in Drosophila Orb2A PLD Relevant for Functional Aggregation in Memory Consolidation"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 90 residues, 10081 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: ???

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSMYNKFVNFICGGLPNLNL NKPPQLHQQQHQQQHQQHQQ HQQQQQLHQHQQQLSPNLSA LHHHHQQQQQLRESGGSHSP SSPGGGGGGS

Data sets:
Data typeCount
13C chemical shifts485
15N chemical shifts167
1H chemical shifts254

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Orb2A PLD1

Entities:

Entity 1, Orb2A PLD 90 residues - 10081 Da.

G1 & S2 are from cloning and tag cleavage. The WT Orb2A prion-like domain sequence is: M1YNKFVNFICGGLPNLNLNKPPQLHQQQHQQQHQQHQQHQQQQQLHQHQQQLSPNLSALHHHHQQQQQLRESGGSHSPSSPGGGGGGS88.

1   GLYSERMETTYRASNLYSPHEVALASNPHE
2   ILECYSGLYGLYLEUPROASNLEUASNLEU
3   ASNLYSPROPROGLNLEUHISGLNGLNGLN
4   HISGLNGLNGLNHISGLNGLNHISGLNGLN
5   HISGLNGLNGLNGLNGLNLEUHISGLNHIS
6   GLNGLNGLNLEUSERPROASNLEUSERALA
7   LEUHISHISHISHISGLNGLNGLNGLNGLN
8   LEUARGGLUSERGLYGLYSERHISSERPRO
9   SERSERPROGLYGLYGLYGLYGLYGLYSER

Related Database Links:

NCBI Reference Sequence NP_729429.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks