BMRB Entry 50278
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50278
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Title: Canonical and modified dsDNA 12mers
Deposition date: 2020-05-17 Original release date: 2020-06-30
Authors: Dubini, Romeo Cosimo Arrigo; Schon, Alexander; Muller, Markus; Carell, Thomas; Rovo, Petra
Citation: Dubini, Romeo Cosimo Arrigo; Schon, Alexander; Muller, Markus; Carell, Thomas; Rovo, Petra. "Impact of 5-Formylcytosine on the Melting Kineticsof DNA by 1H NMR Chemical Exchange" .
Assembly members:
Mod12 dsDNA, polymer, 12 residues, Formula weight is not available
Can12 dsDNA, polymer, 12 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Mod12 dsDNA: GCGATXGATCGC
Can12 dsDNA: GCGATCGATCGC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 159 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mod12 dsDNA | 1 |
| 2 | Can12 dsDNA | 2 |
Entities:
Entity 1, Mod12 dsDNA 12 residues - Formula weight is not available
| 1 | DG | DC | DG | DA | DT | 5FC | DG | DA | DT | DC | ||||
| 2 | DG | DC |
Entity 2, Can12 dsDNA 12 residues - Formula weight is not available
| 1 | DG | DC | DG | DA | DT | DC | DG | DA | DT | DC | ||||
| 2 | DG | DC |
Samples:
sample_1: Mod12 1.58 mM; D2O 5%; H2O 95%; DSS 25 uM; sodium phosphate 15 mM; sodium azide 0.02%; sodium chloride 25 mM
sample_2: Can12 1.00 mM; D2O 5%; H2O 95%; DSS 25 uM; sodium phosphate 15 mM; sodium azide 0.02%; sodium chloride 25 mM
sample_conditions_1: pH: 7; temperature: 310 K
sample_conditions_2: pH: 7; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 1D 1H | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
Software:
SPARKY v1.414 - chemical shift assignment, peak picking
TOPSPIN v4 - collection, processing
NMR spectrometers:
- Bruker Avance 800 MHz