BMRB Entry 50301

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50301

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Title:
Urea Denatured Staphylococcal Nuclease
Deposition date:
2020-05-29
Original release date:
2020-07-15
Authors:
Mizukami, Takuya; Furuzawa, Shunta; Itoh, Satoru; Segawa, Saho; Ikura, Teikichi; Ihara, Kunio; Okumura, Hisashi; Roder, Heinrich; Maki, Kosuke
Citation:

Citation: Mizukami, Takuya; Furuzawa, Shunta; Itoh, Satoru; Segawa, Saho; Ikura, Teikichi; Ihara, Kunio; Okumura, Hisashi; Roder, Heinrich; Maki, Kosuke. "Energetics and kinetics of substrate analog-coupled staphylococcal nuclease folding revealed by a statistical mechanical approach"  Proc. Natl. Acad. Sci. U.S.A. 117, 19953-19962 (2020).
PubMed: 32737158

Assembly members:

Assembly members:
entity_1, polymer, 149 residues, 16811 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMT7-SN

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ATSTKKLHKEPATLIKAIDG DTVKLMYKGQPMTFRLLLVD TPETKHPKKGVEKYGPEASA FTKKMVENAKKIEVEFDKGQ RTDKYGRGLAYIYADGKMVN EALVRQGLAKVAYVYKPNNT HEQHLRKSEAQAKKEKLNIW SEDNADSGQ

Data sets:
Data typeCount
13C chemical shifts227
15N chemical shifts95
1H chemical shifts95

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Staphylococcal nuclease1

Entities:

Entity 1, Staphylococcal nuclease 149 residues - 16811 Da.

1   ALATHRSERTHRLYSLYSLEUHISLYSGLU
2   PROALATHRLEUILELYSALAILEASPGLY
3   ASPTHRVALLYSLEUMETTYRLYSGLYGLN
4   PROMETTHRPHEARGLEULEULEUVALASP
5   THRPROGLUTHRLYSHISPROLYSLYSGLY
6   VALGLULYSTYRGLYPROGLUALASERALA
7   PHETHRLYSLYSMETVALGLUASNALALYS
8   LYSILEGLUVALGLUPHEASPLYSGLYGLN
9   ARGTHRASPLYSTYRGLYARGGLYLEUALA
10   TYRILETYRALAASPGLYLYSMETVALASN
11   GLUALALEUVALARGGLNGLYLEUALALYS
12   VALALATYRVALTYRLYSPROASNASNTHR
13   HISGLUGLNHISLEUARGLYSSERGLUALA
14   GLNALALYSLYSGLULYSLEUASNILETRP
15   SERGLUASPASNALAASPSERGLYGLN

Related Database Links:

PDB 1EY0

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SPARKY: Backbone or all simulated peaks