BMRB Entry 50305
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50305
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Title: Assignment NaK2K Y66F channel PubMed: 34090923
Deposition date: 2020-06-05 Original release date: 2021-06-15
Authors: Hendriks, Kitty; Oester, Carl; Shi, Chaowei; Sun, Han; Lange, Adam
Citation: Hendriks, Kitty; Oester, Carl; Shi, Chaowei; Sun, Han; Lange, Adam. "Sodium ions do not stabilize the selectivity filter of a potassium channel." J. Mol. Biol. 433, 167091-167091 (2021).
Assembly members:
entity_1, polymer, 107 residues, Formula weight is not available
Natural source: Common Name: Bacillus cereus Taxonomy ID: 1396 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus cereus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: MAKDKEFQVLFVLTILTLIS
GTIFYSTVEGLRPIDALYFS
VVTLTTVGFGDFSPQTDFGK
IFTILYIFIGIGLVFGFIHK
LAVNVQLPSILSNLVPRGSR
SHHHHHH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 371 |
15N chemical shifts | 103 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | NaK1K, chain 1 | 1 |
2 | NaK1K, chain 2 | 1 |
3 | NaK1K, chain 3 | 1 |
4 | NaK1K, chain 4 | 1 |
Entities:
Entity 1, NaK1K, chain 1 107 residues - Formula weight is not available
1 | MET | ALA | LYS | ASP | LYS | GLU | PHE | GLN | VAL | LEU | ||||
2 | PHE | VAL | LEU | THR | ILE | LEU | THR | LEU | ILE | SER | ||||
3 | GLY | THR | ILE | PHE | TYR | SER | THR | VAL | GLU | GLY | ||||
4 | LEU | ARG | PRO | ILE | ASP | ALA | LEU | TYR | PHE | SER | ||||
5 | VAL | VAL | THR | LEU | THR | THR | VAL | GLY | PHE | GLY | ||||
6 | ASP | PHE | SER | PRO | GLN | THR | ASP | PHE | GLY | LYS | ||||
7 | ILE | PHE | THR | ILE | LEU | TYR | ILE | PHE | ILE | GLY | ||||
8 | ILE | GLY | LEU | VAL | PHE | GLY | PHE | ILE | HIS | LYS | ||||
9 | LEU | ALA | VAL | ASN | VAL | GLN | LEU | PRO | SER | ILE | ||||
10 | LEU | SER | ASN | LEU | VAL | PRO | ARG | GLY | SER | ARG | ||||
11 | SER | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: NaK1K tetramer, [U-98% 13C; U-98% 15N], 4.6 mg; potassium chloride 50 mM; TRIS 20 mM; E. coli total lipid extract 2.3 mg
sample_2: NaK1K tetramer, [U-98% 13C; U-98% 15N], 4.6 mg; sodium chloride 50 mM; TRIS 20 mM; E. coli total lipid extract 2.3 mg
sample_conditions_1: ionic strength: 50 mM; pH: 8; pressure: 1 atm; temperature: 283 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D NCA | sample_1 | isotropic | sample_conditions_1 |
2D NCO | sample_1 | isotropic | sample_conditions_1 |
2D 13C/13C PDSD | sample_1 | isotropic | sample_conditions_1 |
3D NCACO | sample_1 | isotropic | sample_conditions_1 |
3D NCACB | sample_1 | isotropic | sample_conditions_1 |
3D NCOCA | sample_1 | isotropic | sample_conditions_1 |
3D CANCO | sample_1 | isotropic | sample_conditions_1 |
3D NCOCACB | sample_1 | isotropic | sample_conditions_1 |
3D NCACX | sample_1 | isotropic | sample_conditions_1 |
2D NCA | sample_2 | isotropic | sample_conditions_1 |
2D NCO | sample_2 | isotropic | sample_conditions_1 |
2D 13C/13C PDSD | sample_2 | isotropic | sample_conditions_1 |
3D NCACO | sample_2 | isotropic | sample_conditions_1 |
3D NCACB | sample_2 | isotropic | sample_conditions_1 |
3D NCOCA | sample_2 | isotropic | sample_conditions_1 |
3D CANCO | sample_2 | isotropic | sample_conditions_1 |
3D NCOCACB | sample_2 | isotropic | sample_conditions_1 |
3D NCACX | sample_2 | isotropic | sample_conditions_1 |
Software:
CcpNmr Analysis - chemical shift assignment
TOPSPIN - collection, processing
NMR spectrometers:
- Bruker Avance 700 MHz