BMRB Entry 5032
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5032
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Title: 1H, 13C and 15N Resonance Assignments of the DNA Binding Domain of the Human Forkhead Transcription Factor AFX PubMed: 10921784
Deposition date: 2001-05-28 Original release date: 2001-06-05
Authors: Weigelt, Johan; Climent, Isabel; Dahlman-Wright, K.; Wikstrom, Mats
Citation: Weigelt, Johan; Climent, Isabel; Dahlman-Wright, K.; Wikstrom, Mats. "Letter to the Editor: 1H, 13C and 15N Resonance Assignments of the DNA Binding Domain of the Human Forkhead Transcription Factor AFX" J. Biomol. NMR 17, 181-182 (2000).
Assembly members:
AFX DNA binding domain, polymer, 108 residues, Formula weight is not available
Insulin response element from IGFBP-1 promoter, polymer, 16 residues, Formula weight is not available
Insulin response element from IGFBP-1 promoter, polymer, 16 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
AFX DNA binding domain: MRGSHHHHHHGSRRNAWGNQ
SYAELISQAIESAPEKRLTL
AQIYEWMVRTVPYFKDKGDS
NSSAGWKNSIRHNLSLHSKF
IKVHNEATGKSSWWMLNPEG
GKSGKAPR
Insulin response element from IGFBP-1 promoter: CAAGCAAAACAAACCA
Insulin response element from IGFBP-1 promoter: TGGTTTGTTTTGCTTG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 75 |
| 13C chemical shifts | 147 |
| 15N chemical shifts | 75 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AFX DNA binding domain | 1 |
| 2 | 16mer insulin response sequence, strand 1 | 2 |
| 3 | 16mer insulin response sequence, strand 2 | 3 |
Entities:
Entity 1, AFX DNA binding domain 108 residues - Formula weight is not available
| 1 | MET | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | GLY | SER | ARG | ARG | ASN | ALA | TRP | GLY | ASN | GLN | ||||
| 3 | SER | TYR | ALA | GLU | LEU | ILE | SER | GLN | ALA | ILE | ||||
| 4 | GLU | SER | ALA | PRO | GLU | LYS | ARG | LEU | THR | LEU | ||||
| 5 | ALA | GLN | ILE | TYR | GLU | TRP | MET | VAL | ARG | THR | ||||
| 6 | VAL | PRO | TYR | PHE | LYS | ASP | LYS | GLY | ASP | SER | ||||
| 7 | ASN | SER | SER | ALA | GLY | TRP | LYS | ASN | SER | ILE | ||||
| 8 | ARG | HIS | ASN | LEU | SER | LEU | HIS | SER | LYS | PHE | ||||
| 9 | ILE | LYS | VAL | HIS | ASN | GLU | ALA | THR | GLY | LYS | ||||
| 10 | SER | SER | TRP | TRP | MET | LEU | ASN | PRO | GLU | GLY | ||||
| 11 | GLY | LYS | SER | GLY | LYS | ALA | PRO | ARG |
Entity 2, 16mer insulin response sequence, strand 1 16 residues - Formula weight is not available
| 1 | DC | DA | DA | DG | DC | DA | DA | DA | DA | DC | ||||
| 2 | DA | DA | DA | DC | DC | DA |
Entity 3, 16mer insulin response sequence, strand 2 16 residues - Formula weight is not available
| 1 | DT | DG | DG | DT | DT | DT | DG | DT | DT | DT | ||||
| 2 | DT | DG | DC | DT | DT | DG |
Samples:
sample_1: AFX DNA binding domain, [U-13C; U-15N; U-90% 2H], 0.5 mM; Insulin response element from IGFBP-1 promoter 0.5 mM; Insulin response element from IGFBP-1 promoter 0.5 mM; sodium phospate 20 mM; NaCl 50 mM; MgCl2 2 mM; NaN3 0.02 % w/v
Exp_cond: pH: 6.4; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N TROSY | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
| HNCACB-TROSY | not available | not available | not available |
| HNCA-TROSY | not available | not available | not available |
Software:
nmrPipe - processing
ANSIG - analysis
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA86199 BAE34214 BAG61928 BAG73163 |
| EMBL | CAA63819 CAA72156 |
| GB | AAD42108 AAH99460 AAI06762 AAI39276 AAI39305 |
| REF | NP_001094747 NP_001100413 NP_005929 NP_061259 XP_002720130 |
| SP | P98177 Q9WVH3 |
| TPG | DAA12928 |
| AlphaFold | Q9WVH3 P98177 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts