BMRB Entry 50325

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50325

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Title:
Backbone chemical shift assignment of the FRB domain of mTOR
Deposition date:
2020-06-12
Original release date:
2021-08-20
Authors:
Heimhalt, Maren; Berndt, Alex; Wagstaff, Jane; Perisic, Olga; Maslen, Sarah; Yu, Conny Wing-Heng; Anandapadamanaban, Madhangopal; Masson, Glenn; Boland, Andreas; Johnson, Christopher; McLaughlin, Stephen; Skehel, Mark; Freund, Stefan; Williams, Roger
Citation:

Citation: Heimhalt, Maren; Berndt, Alex; Wagstaff, Jane; Anandapadamanaban, Madhangopal; Perisic, Olga; Maslen, Sarah; McLaughlin, Stephen; Yu, Conny Wing-Heng; Masson, Glenn; Boland, Andreas; Ni, Xiaodan; Yamashita, Keitaro; Murshudov, Garib; Skehel, Mark; Freund, Stefan; Williams, Roger. "Bipartite binding and partial inhibition links DEPTOR and mTOR in a mutually antagonistic embrace"  Elife 10, e68799-e68799 (2021).
PubMed: 34519269

Assembly members:

Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pOPTG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMELIRVAILWHEMWHEG LEEASRLYFGERNVKGMFEV LEPLHAMMERGPQTLKETSF NQAYGRDLMEAQEWCRKYMK SGNVKDLTQAWDLYYHVFRR ISKQ

Data sets:
Data typeCount
13C chemical shifts294
15N chemical shifts93
1H chemical shifts93

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1mTOR-FRB1

Entities:

Entity 1, mTOR-FRB 104 residues - Formula weight is not available

This construct contains a cloning scar at the N-terminus (GSHM) the construct covers residues 2015 to 2114 of the published DEPTOR sequence.

1   GLYSERHISMETGLULEUILEARGVALALA
2   ILELEUTRPHISGLUMETTRPHISGLUGLY
3   LEUGLUGLUALASERARGLEUTYRPHEGLY
4   GLUARGASNVALLYSGLYMETPHEGLUVAL
5   LEUGLUPROLEUHISALAMETMETGLUARG
6   GLYPROGLNTHRLEULYSGLUTHRSERPHE
7   ASNGLNALATYRGLYARGASPLEUMETGLU
8   ALAGLNGLUTRPCYSARGLYSTYRMETLYS
9   SERGLYASNVALLYSASPLEUTHRGLNALA
10   TRPASPLEUTYRTYRHISVALPHEARGARG
11   ILESERLYSGLN

Related Database Links:

UniprotKB P42345

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks