BMRB Entry 50326

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50326

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Title:
Backbone chemical shift assignment of the linker region of DEPTOR
Deposition date:
2020-06-12
Original release date:
2021-08-20
Authors:
Heimhalt, Maren; Berndt, Alex; Wagstaff, Jane; Perisic, Olga; Maslen, Sarah; Yu, Conny Wing-Heng; Anandapadamanaban, Madhangopal; Masson, Glenn; Boland, Andreas; Johnson, Christopher; McLaughlin, Stephen; Skehel, Mark; Freund, Stefan; Williams, Roger
Citation:

Citation: Heimhalt, Maren; Berndt, Alex; Wagstaff, Jane; Anandapadamanaban, Madhangopal; Perisic, Olga; Maslen, Sarah; McLaughlin, Stephen; Yu, Conny Wing-Heng; Masson, Glenn; Boland, Andreas; Ni, Xiaodan; Yamashita, Keitaro; Murshudov, Garib; Skehel, Mark; Freund, Stefan; Williams, Roger. "Bipartite binding and partial inhibition links DEPTOR and mTOR in a mutually antagonistic embrace"  Elife 10, e68799-e68799 (2021).
PubMed: 34519269

Assembly members:

Assembly members:
entity_1, polymer, 99 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pOPTH

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMELLNEKSPSSQETHDS PFCLRKQSHDNRKSTSFMSV SPSKEIKIVSAVRRSSMSSC GSSGYFSSSPTLSSSPPVLC NPKSVLKRPVTSEELLTPG

Data sets:
Data typeCount
13C chemical shifts285
15N chemical shifts94
1H chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DEPTOR Linker1

Entities:

Entity 1, DEPTOR Linker 99 residues - Formula weight is not available

This construct contains a cloning scar at the N-terminus (GSH) the construct covers residues 228 to 323 of the published DEPTOR sequence.

1   GLYSERHISMETGLULEULEUASNGLULYS
2   SERPROSERSERGLNGLUTHRHISASPSER
3   PROPHECYSLEUARGLYSGLNSERHISASP
4   ASNARGLYSSERTHRSERPHEMETSERVAL
5   SERPROSERLYSGLUILELYSILEVALSER
6   ALAVALARGARGSERSERMETSERSERCYS
7   GLYSERSERGLYTYRPHESERSERSERPRO
8   THRLEUSERSERSERPROPROVALLEUCYS
9   ASNPROLYSSERVALLEULYSARGPROVAL
10   THRSERGLUGLULEULEUTHRPROGLY

Related Database Links:

UniprotKB Q8TB45

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks