BMRB Entry 50334

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50334

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Title:
1H, 13C, and 15N backbone chemical shift assignments of the nucleic acid-binding domain of coronavirus-2 non-structural protein 3e
Deposition date:
2020-06-21
Original release date:
2020-06-27
Authors:
Korn, Sophie; Dhamotharan, Karthikeyan; Furtig, Boris; Hengesbach, Martin; Lohr, Frank; Qureshi, Nusrat; Richter, Christian; Saxena, Krishna; Schwalbe, Harald; Tants, Jan-Niklas; Weigand, Julia; Wohnert, Jens; Schlundt, Andreas
Citation:

Citation: Korn, Sophie; Dhamotharan, Karthikeyan; Furtig, Boris; Hengesbach, Martin; Lohr, Frank; Qureshi, Nusrat; Richter, Christian; Saxena, Krishna; Schwalbe, Harald; Tants, Jan-Niklas; Weigand, Julia; Wohnert, Jens; Schlundt, Andreas. "1H, 13C, and 15N backbone chemical shift assignments of the nucleic acid-binding domain of SARS-CoV-2 non-structural protein 3e"  Biomol. NMR Assign. 14, 329-333 (2020).
PubMed: 32770392

Assembly members:

Assembly members:
entity_1, polymer, 120 residues, 13745 Da.

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKM263

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGYFTEQPIDLVPNQPYP NASFDNFKFVCDNIKFADDL NQLTGYKKPASRELKVTFFP DLNGDVVAIDYKHYTPSFKK GAKLLHKPIVWHVNNATNKA TYKPNTWCIRCLWSTKPVET

Data sets:
Data typeCount
13C chemical shifts350
15N chemical shifts115
1H chemical shifts122

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Nsp3e1

Entities:

Entity 1, Nsp3e 120 residues - 13745 Da.

GAMG is an artificial overhang from TEV cleavage.

1   GLYALAMETGLYTYRPHETHRGLUGLNPRO
2   ILEASPLEUVALPROASNGLNPROTYRPRO
3   ASNALASERPHEASPASNPHELYSPHEVAL
4   CYSASPASNILELYSPHEALAASPASPLEU
5   ASNGLNLEUTHRGLYTYRLYSLYSPROALA
6   SERARGGLULEULYSVALTHRPHEPHEPRO
7   ASPLEUASNGLYASPVALVALALAILEASP
8   TYRLYSHISTYRTHRPROSERPHELYSLYS
9   GLYALALYSLEULEUHISLYSPROILEVAL
10   TRPHISVALASNASNALATHRASNLYSALA
11   THRTYRLYSPROASNTHRTRPCYSILEARG
12   CYSLEUTRPSERTHRLYSPROVALGLUTHR

Related Database Links:

NCBI YP_009725299.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks