BMRB Entry 50353

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR50353

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Title:
1H, 13C, 15N chemical shift assignment of NTD MaSp2 from Nephila clavipes
Deposition date:
2020-06-23
Original release date:
2020-08-11
Authors:
Oktaviani, Nur Alia; Malay, Ali D; Matsugami, Akimasa; Hayashi, Fumiaki; Numata, Keiji
Citation:

Citation: Oktaviani, Nur Alia; Malay, Ali D; Matsugami, Akimasa; Hayashi, Fumiaki; Numata, Keiji. "Nearly complete 1H, 13C and 15N chemical shift assignment of monomeric form of N-terminal domain of Nephila clavipes major ampullate spidroin 2"  Biomol. NMR Assignments 14, 335-338 (2020).
PubMed: 32767002

Assembly members:

Assembly members:
entity_1, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Nephila clavipes   Taxonomy ID: 2585209   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Nephila clavipes

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMAQARSPWSDTATADAF IQNFLAAVSGSGAFTSDQLD DMSTIGDTIMSAMDKMARSN KSSQHKLQALNMAFASSMAE IAAVEQGGMSMAVKTNAIVD GLNSAFYMTTGAANPQFVNE MRSLISMISAASANEVSY

Data sets:
Data typeCount
13C chemical shifts573
15N chemical shifts143
1H chemical shifts881

Additional metadata:

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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal domain of Major Ampullate Spidroin 21

Entities:

Entity 1, N-terminal domain of Major Ampullate Spidroin 2 138 residues - Formula weight is not available

1   GLYSERHISMETALAGLNALAARGSERPRO
2   TRPSERASPTHRALATHRALAASPALAPHE
3   ILEGLNASNPHELEUALAALAVALSERGLY
4   SERGLYALAPHETHRSERASPGLNLEUASP
5   ASPMETSERTHRILEGLYASPTHRILEMET
6   SERALAMETASPLYSMETALAARGSERASN
7   LYSSERSERGLNHISLYSLEUGLNALALEU
8   ASNMETALAPHEALASERSERMETALAGLU
9   ILEALAALAVALGLUGLNGLYGLYMETSER
10   METALAVALLYSTHRASNALAILEVALASP
11   GLYLEUASNSERALAPHETYRMETTHRTHR
12   GLYALAALAASNPROGLNPHEVALASNGLU
13   METARGSERLEUILESERMETILESERALA
14   ALASERALAASNGLUVALSERTYR

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