BMRB Entry 50379

Name: N SARS-CoV-2 SR-rich motif (182-197)
Published: 2021-07-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50379

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: N SARS-CoV-2 SR-rich motif (182-197) PubMed: 33247108

Deposition date: 2020-07-07 Original release date: 2021-07-27

Authors: Ibanez de Opakua, Alain; Savastano, Adriana; Zweckstetter, Markus

Citation: Savastano, Adriana; Ibanez de Opakua, Alain; Rankovic, Marija; Zweckstetter, Markus. "Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates" Nat. Commun. 11, 6041-6041 (2020).

Assembly members:
entity_1, polymer, 18 residues, Formula weight is not available

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: XASSRSSSRSRNSSRNSX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	32
15N chemical shifts	16
1H chemical shifts	32

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SR	1

Entities:

Entity 1, SR 18 residues - Formula weight is not available

1

ACE

ALA

SER

ARG

SER

ARG

SER

2

ARG

ASN

SER

ARG

ASN

SER

NH2

Samples:

sample_1: SR 3 mM; D2O, [U-2H], 5%; sodium phosphate 50 mM; sodium azide 0.01%

sample_conditions_1: ionic strength: 0.092 M; pH: 6.8; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection, processing

NMRFAM-SPARKY - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts