BMRB Entry 50386

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50386

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Title:
Phosphorylated tEIN backbone and I/L/V methyl resonance assignment
Deposition date:
2020-07-10
Original release date:
2020-07-15
Authors:
Purslow, Jeffrey; Dotas, Rochelle; Sivo, Valeria; Venditti, Vincenzo
Citation:

Citation: Purslow, Jeffrey; Thimmesch, Jolene; Sivo, Valeria; Nguyen, Trang; Khatiwada, Balabhadra; Dotas, Rochelle; Venditti, Vincenzo. "Phosphorylation and single-point mutation effects on the conformational dynamics of EIN"  Front. Mol. Biosci. 8, 699203-699203 (2021).
PubMed: 34307459

Assembly members:

Assembly members:
entity_1, polymer, 248 residues, Formula weight is not available
PHOSPHOENOLPYRUVATE, non-polymer, 168.042 Da.

Natural source:

Natural source:   Common Name: Caldanaerobacter subterraneus   Taxonomy ID: 911092   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Caldanaerobacter subterraneus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MLKGVAASPGIAIGKAFLYT KEKVTINVEKIEESKVEEEI AKFRKALEVTQEEIEKIKEK ALKEFGKEKAEIFEAHLMLA SDPELIEGVENMIKTELVTA DNAVNKVIEQNASVMESLND EYLKERAVDLRDVGNRIIEN LLGVKSVNLSDLEEEVVVIA RDLTPSDTATMKKEMVLGFA TDVGGITSXTAIMARSLEIP AVVGLGNVTSQVKAGDLVIV DGLEGIVIVNPDEKTVEDYK SKKESYEK

Data sets:
Data typeCount
13C chemical shifts825
15N chemical shifts466
1H chemical shifts792

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Phosphorylated tEIN1
2PEP2

Entities:

Entity 1, Phosphorylated tEIN 248 residues - Formula weight is not available

1   METLEULYSGLYVALALAALASERPROGLY
2   ILEALAILEGLYLYSALAPHELEUTYRTHR
3   LYSGLULYSVALTHRILEASNVALGLULYS
4   ILEGLUGLUSERLYSVALGLUGLUGLUILE
5   ALALYSPHEARGLYSALALEUGLUVALTHR
6   GLNGLUGLUILEGLULYSILELYSGLULYS
7   ALALEULYSGLUPHEGLYLYSGLULYSALA
8   GLUILEPHEGLUALAHISLEUMETLEUALA
9   SERASPPROGLULEUILEGLUGLYVALGLU
10   ASNMETILELYSTHRGLULEUVALTHRALA
11   ASPASNALAVALASNLYSVALILEGLUGLN
12   ASNALASERVALMETGLUSERLEUASNASP
13   GLUTYRLEULYSGLUARGALAVALASPLEU
14   ARGASPVALGLYASNARGILEILEGLUASN
15   LEULEUGLYVALLYSSERVALASNLEUSER
16   ASPLEUGLUGLUGLUVALVALVALILEALA
17   ARGASPLEUTHRPROSERASPTHRALATHR
18   METLYSLYSGLUMETVALLEUGLYPHEALA
19   THRASPVALGLYGLYILETHRSERNEPTHR
20   ALAILEMETALAARGSERLEUGLUILEPRO
21   ALAVALVALGLYLEUGLYASNVALTHRSER
22   GLNVALLYSALAGLYASPLEUVALILEVAL
23   ASPGLYLEUGLUGLYILEVALILEVALASN
24   PROASPGLULYSTHRVALGLUASPTYRLYS
25   SERLYSLYSGLUSERTYRGLULYS

Entity 2, PEP - C3 H5 O6 P - 168.042 Da.

1   PEP

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks