BMRB Entry 50398
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50398
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Title: Backbone/side_chain assignment of human FOXO4 86_207 PubMed: 34320339
Deposition date: 2020-07-21 Original release date: 2021-08-11
Authors: Bourgeois, Benjamin; Madl, Tobias
Citation: Bourgeois, Benjamin; Gui, Tianshu; Hoogeboom, Diana; Hocking, Henry; Richter, Gesa; Spreitzer, Emil; Viertler, Martin; Richter, Klaus; Madl, Tobias; Burgering, Boudewijn. "Multiple regulatory intrinsically disordered motifs control FOXO4 transcription factor binding and function" Cell Rep. 36, 109446-109446 (2021).
Assembly members:
entity_1, polymer, 125 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETZ2_1a
Entity Sequences (FASTA):
entity_1: GAMGAVTGPRKGGSRRNAWG
NQSYAELISQAIESAPEKRL
TLAQIYEWMVRTVPYFKDKG
DSNSSAGWKNSIRHNLSLHS
KFIKVHNEATGKSSWWMLNP
EGGKSGKAPRRRAASMDSSS
KLLRG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 334 |
| 15N chemical shifts | 123 |
| 1H chemical shifts | 657 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human FOXO4 86_207 | 1 |
Entities:
Entity 1, human FOXO4 86_207 125 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLY | ALA | VAL | THR | GLY | PRO | ARG | ||||
| 2 | LYS | GLY | GLY | SER | ARG | ARG | ASN | ALA | TRP | GLY | ||||
| 3 | ASN | GLN | SER | TYR | ALA | GLU | LEU | ILE | SER | GLN | ||||
| 4 | ALA | ILE | GLU | SER | ALA | PRO | GLU | LYS | ARG | LEU | ||||
| 5 | THR | LEU | ALA | GLN | ILE | TYR | GLU | TRP | MET | VAL | ||||
| 6 | ARG | THR | VAL | PRO | TYR | PHE | LYS | ASP | LYS | GLY | ||||
| 7 | ASP | SER | ASN | SER | SER | ALA | GLY | TRP | LYS | ASN | ||||
| 8 | SER | ILE | ARG | HIS | ASN | LEU | SER | LEU | HIS | SER | ||||
| 9 | LYS | PHE | ILE | LYS | VAL | HIS | ASN | GLU | ALA | THR | ||||
| 10 | GLY | LYS | SER | SER | TRP | TRP | MET | LEU | ASN | PRO | ||||
| 11 | GLU | GLY | GLY | LYS | SER | GLY | LYS | ALA | PRO | ARG | ||||
| 12 | ARG | ARG | ALA | ALA | SER | MET | ASP | SER | SER | SER | ||||
| 13 | LYS | LEU | LEU | ARG | GLY |
Samples:
sample_1: human FOXO4, [U-99% 13C; U-99% 15N], 500 uM; TRIS 50 mM; sodium chloride 150 mM; TCEP 2 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
TOPSPIN - processing
NMR spectrometers:
- Bruker AVANCE III 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts