BMRB Entry 50402

Name: hFOXO4_465-500
Published: 2021-08-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50402

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: hFOXO4_465-500 PubMed: 34320339

Deposition date: 2020-07-22 Original release date: 2021-08-11

Authors: Bourgeois, Benjamin; Madl, Tobias

Citation: Bourgeois, Benjamin; Gui, Tianshu; Hoogeboom, Diana; Hocking, Henry; Richter, Gesa; Spreitzer, Emil; Viertler, Martin; Richter, Klaus; Madl, Tobias; Burgering, Boudewijn. "Multiple regulatory intrinsically disordered motifs control FOXO4 transcription factor binding and function" Cell Rep. 36, 109446-109446 (2021).

Assembly members:
entity_1, polymer, 36 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETZ1a

Entity Sequences (FASTA):
entity_1: RMPQDLDLDMYMENLECDMD NIISDLMDEGEGLDFN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	63
15N chemical shifts	35
1H chemical shifts	35

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human hFOXO4 465-500	1

Entities:

Entity 1, human hFOXO4 465-500 36 residues - Formula weight is not available

1

ARG

MET

PRO

GLN

ASP

LEU

ASP

LEU

ASP

MET

2

TYR

MET

GLU

ASN

LEU

GLU

CYS

ASP

MET

ASP

3

ASN

ILE

SER

ASP

LEU

MET

ASP

GLU

GLY

4

GLU

GLY

LEU

ASP

PHE

ASN

Samples:

sample_1: FOXO4_465-500, [U-99% 13C; U-99% 15N], 500 uM; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 5 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

TOPSPIN - processing

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts