BMRB Entry 50414
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50414
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Title: A10 nanobody protein backbone assignation PubMed: 25223348
Deposition date: 2020-07-29 Original release date: 2020-08-11
Authors: Kovacic, Matic; Ilc, Gregor; de Marco, Ario
Citation: Djender, Selma; Schneider, Aurelie; Beugnet, Anne; Crepin, Ronan; Desrumeaux, Klervi Even; Romani, Chiara; Moutel, Sandrine; Perez, Franck; de Marco, Ario. "Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies." Microb. Cell Fact. 13, 140-140 (2014).
Assembly members:
entity_1, polymer, 137 residues, Formula weight is not available
Natural source: Common Name: llama Taxonomy ID: 9844 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lama glama
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM14
Entity Sequences (FASTA):
entity_1: MAEVQLQASGGGFVQPGGSL
RLSCAASGATSNISNMGWFR
QAPGKEREFVSAISRAESRP
LYYADSVKGRFTISRDNSKN
TVYLQMNSLRAEDTATYYCA
YMPLVRHKAYWGQGTQVTVS
SAAAHHHHHHGAAEPEA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 188 |
| 15N chemical shifts | 100 |
| 1H chemical shifts | 100 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | nanobody | 1 |
Entities:
Entity 1, nanobody 137 residues - Formula weight is not available
| 1 | MET | ALA | GLU | VAL | GLN | LEU | GLN | ALA | SER | GLY | ||||
| 2 | GLY | GLY | PHE | VAL | GLN | PRO | GLY | GLY | SER | LEU | ||||
| 3 | ARG | LEU | SER | CYS | ALA | ALA | SER | GLY | ALA | THR | ||||
| 4 | SER | ASN | ILE | SER | ASN | MET | GLY | TRP | PHE | ARG | ||||
| 5 | GLN | ALA | PRO | GLY | LYS | GLU | ARG | GLU | PHE | VAL | ||||
| 6 | SER | ALA | ILE | SER | ARG | ALA | GLU | SER | ARG | PRO | ||||
| 7 | LEU | TYR | TYR | ALA | ASP | SER | VAL | LYS | GLY | ARG | ||||
| 8 | PHE | THR | ILE | SER | ARG | ASP | ASN | SER | LYS | ASN | ||||
| 9 | THR | VAL | TYR | LEU | GLN | MET | ASN | SER | LEU | ARG | ||||
| 10 | ALA | GLU | ASP | THR | ALA | THR | TYR | TYR | CYS | ALA | ||||
| 11 | TYR | MET | PRO | LEU | VAL | ARG | HIS | LYS | ALA | TYR | ||||
| 12 | TRP | GLY | GLN | GLY | THR | GLN | VAL | THR | VAL | SER | ||||
| 13 | SER | ALA | ALA | ALA | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 14 | GLY | ALA | ALA | GLU | PRO | GLU | ALA |
Samples:
sample_1: A10 nanobody, [U-99% 13C; U-99% 15N], 0.25 mM; sodium chloride 137 mM; potassium chloride 2.7 mM; disodium phosphate 10 mM; monosodium phosphate 1.8 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7.3; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA v1.9.1.7 - chemical shift assignment
NMRFAM-SPARKY - data analysis
VNMR - collection
NMRPipe - processing
NMR spectrometers:
- Agilent Technologies VNMRS 800 MHz 18.8T MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts