BMRB Entry 50425
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50425
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Title: Influenza A matrix protein 1 PubMed: 33516724
Deposition date: 2020-08-05 Original release date: 2021-01-26
Authors: Mohd-Kipli, Faiz; Claridge, Jolyon; Schnell, Jason
Citation: Mohd-Kipli, Faiz; Claridge, Jolyon; Habjanic, Jelena; Jiang, Alex; Schnell, Jason. "Conformational triggers associated with influenza matrix protein 1 polymerization" J. Biol. Chem. 296, 100316-100316 (2021).
Assembly members:
entity_1, polymer, 182 residues, Formula weight is not available
Natural source: Common Name: Influenza A virus Taxonomy ID: 11320 Superkingdom: Viruses Kingdom: not available Genus/species: Alphainfluenzavirus Influenza A
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCOLD II
Entity Sequences (FASTA):
entity_1: MSLLTEVETYVLSIIPSGPL
KAEIAQRLESVFAGKNTDLE
ALMEWLKTRPILSPLTKGIL
GFVFTLTVPSERGLQRRRFV
QNALNGNGDPNNMDRAVKLY
KKLKREITFHGAKEVSLSYS
TGALASCMGLIYNRMGTVTT
EAAFGLVCATCEQIADSQHR
SHRQLEVLFQGPGSHHHHHH
HH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 394 |
| 15N chemical shifts | 142 |
| 1H chemical shifts | 142 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Influenza A virus matrix 2 protein | 1 |
Entities:
Entity 1, Influenza A virus matrix 2 protein 182 residues - Formula weight is not available
Residues 1-164 of matrix protein 1 derived from influenza strain A/England/250/2009(H1N1). C-terminal tag includes 3C protease cut site, linker, and 8-histidine affinity tag.
| 1 | MET | SER | LEU | LEU | THR | GLU | VAL | GLU | THR | TYR | ||||
| 2 | VAL | LEU | SER | ILE | ILE | PRO | SER | GLY | PRO | LEU | ||||
| 3 | LYS | ALA | GLU | ILE | ALA | GLN | ARG | LEU | GLU | SER | ||||
| 4 | VAL | PHE | ALA | GLY | LYS | ASN | THR | ASP | LEU | GLU | ||||
| 5 | ALA | LEU | MET | GLU | TRP | LEU | LYS | THR | ARG | PRO | ||||
| 6 | ILE | LEU | SER | PRO | LEU | THR | LYS | GLY | ILE | LEU | ||||
| 7 | GLY | PHE | VAL | PHE | THR | LEU | THR | VAL | PRO | SER | ||||
| 8 | GLU | ARG | GLY | LEU | GLN | ARG | ARG | ARG | PHE | VAL | ||||
| 9 | GLN | ASN | ALA | LEU | ASN | GLY | ASN | GLY | ASP | PRO | ||||
| 10 | ASN | ASN | MET | ASP | ARG | ALA | VAL | LYS | LEU | TYR | ||||
| 11 | LYS | LYS | LEU | LYS | ARG | GLU | ILE | THR | PHE | HIS | ||||
| 12 | GLY | ALA | LYS | GLU | VAL | SER | LEU | SER | TYR | SER | ||||
| 13 | THR | GLY | ALA | LEU | ALA | SER | CYS | MET | GLY | LEU | ||||
| 14 | ILE | TYR | ASN | ARG | MET | GLY | THR | VAL | THR | THR | ||||
| 15 | GLU | ALA | ALA | PHE | GLY | LEU | VAL | CYS | ALA | THR | ||||
| 16 | CYS | GLU | GLN | ILE | ALA | ASP | SER | GLN | HIS | ARG | ||||
| 17 | SER | HIS | ARG | GLN | LEU | GLU | VAL | LEU | PHE | GLN | ||||
| 18 | GLY | PRO | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 19 | HIS | HIS |
Samples:
sample_1: Influenza A virus matrix protein 1, [U-99% 13C; U-99% 15N], 0.175 mM; TRIS 20 mM; NaCl 50 mM; EDTA 1 mM; beta-mercaptoethanol 2 mM
sample_conditions_1: ionic strength: 0.120 M; pH: 5.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCOCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCOCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v8.7 - processing
CARA v1.9.1.7 - chemical shift assignment
NMR spectrometers:
- Bruker Avance III HD 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts