BMRB Entry 50426
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50426
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Title: FOXP2_FH_504-594 PubMed: 33284517
Deposition date: 2020-08-06 Original release date: 2021-06-30
Authors: Richter, Gesa; Madl, Tobias
Citation: Richter, Gesa; Gui, Tianshu; Bourgeois, Benjamin; Koyani, Chintan; Ulz, Peter; Heitzer, Ellen; von Lewinski, Dirk; Burgering, Boudewijn; Malle, Ernst; Madl, Tobias. "b-catenin regulates FOXP2 transcriptional activity via multiple binding sites" FEBS J. 288, 3261-3284 (2021).
Assembly members:
entity_1, polymer, 91 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: petZ2_1a
Entity Sequences (FASTA):
entity_1: RPPFTYATLIRQAIMESSDR
QLTLNEIYSWFTRTFAYFRR
NAATWKNAVRHNLSLHKCFV
RVENVKGAVWTVDEVEYQKR
RSQKITGSPTL
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 79 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FOXP2_FH_504-594 | 1 |
Entities:
Entity 1, FOXP2_FH_504-594 91 residues - Formula weight is not available
| 1 | ARG | PRO | PRO | PHE | THR | TYR | ALA | THR | LEU | ILE | ||||
| 2 | ARG | GLN | ALA | ILE | MET | GLU | SER | SER | ASP | ARG | ||||
| 3 | GLN | LEU | THR | LEU | ASN | GLU | ILE | TYR | SER | TRP | ||||
| 4 | PHE | THR | ARG | THR | PHE | ALA | TYR | PHE | ARG | ARG | ||||
| 5 | ASN | ALA | ALA | THR | TRP | LYS | ASN | ALA | VAL | ARG | ||||
| 6 | HIS | ASN | LEU | SER | LEU | HIS | LYS | CYS | PHE | VAL | ||||
| 7 | ARG | VAL | GLU | ASN | VAL | LYS | GLY | ALA | VAL | TRP | ||||
| 8 | THR | VAL | ASP | GLU | VAL | GLU | TYR | GLN | LYS | ARG | ||||
| 9 | ARG | SER | GLN | LYS | ILE | THR | GLY | SER | PRO | THR | ||||
| 10 | LEU |
Samples:
sample_1: FOXP2_FH_504-594, [U-99% 13C; U-99% 15N], 500 uM; sodium phosphate 50 mM; sodium chloride 150 mM; beta-mercaptoethanol 2 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - data analysis
TOPSPIN - collection, processing
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts