BMRB Entry 50437

Title:
Mutation that removes disulfide in HdeA results in an unfolded protein that gains structure at low pH
Deposition date:
2020-08-14
Original release date:
2020-11-02
Authors:
Aguirre-Cardenas, M. Imex; Geddes-Buehre, Dane; Crowhurst, Karin
Citation:

Citation: Aguirre-Cardenas, M. Imex; Geddes-Buehre, Dane; Crowhurst, Karin. "Removal of disulfide from acid stress chaperone HdeA does not wholly eliminate structure or function at low pH"  Biochem. Biophys. Rep. 27, 101064-101064 (2021).

Assembly members:

Assembly members:
entity_1, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts433
15N chemical shifts164
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HdeA-C18S-C66S1

Entities:

Entity 1, HdeA-C18S-C66S 89 residues - Formula weight is not available

1   ALAASPALAGLNLYSALAALAASPASNLYS
2   LYSPROVALASNSERTRPTHRSERGLUASP
3   PHELEUALAVALASPGLUSERPHEGLNPRO
4   THRALAVALGLYPHEALAGLUALALEUASN
5   ASNLYSASPLYSPROGLUASPALAVALLEU
6   ASPVALGLNGLYILEALATHRVALTHRPRO
7   ALAILEVALGLNALASERTHRGLNASPLYS
8   GLNALAASNPHELYSASPLYSVALLYSGLY
9   GLUTRPASPLYSILELYSLYSASPMET

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks