BMRB Entry 50438
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50438
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Title: H2A and H2B tails in nucleosome PubMed: 34153285
Deposition date: 2020-08-17 Original release date: 2021-06-30
Authors: Ohtomo, Hideaki; Kurita, Jun-ichi; Nishimura, Yoshifumi
Citation: Ohtomo, Hideaki; Kurita, Jun-Ichi; Sakuraba, Shun; Li, Zhenhai; Arimura, Yasuhiro; Wakamori, Masatoshi; Tsunaka, Yasuo; Umehara, Takashi; Kurumizaka, Hitoshi; Kono, Hidetoshi; Nishimura, Yoshifumi. "The N-terminal tails of histones H2A and H2B adopt two distinct conformations in the nucleosome with contact and reduced contact to DNA." J. Mol. Biol. 433, 167110-167110 (2021).
Assembly members:
entity_1, polymer, 133 residues, Formula weight is not available
entity_2, polymer, 129 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET23b
Entity Sequences (FASTA):
entity_1: GPGMSGRGKQGGKARAKAKT
RSSRAGLQFPVGRVHRLLRK
GNYSERVGAGAPVYLAAVLE
YLTAEILELAGNAARDNKKT
RIIPRHLQLAIRNDEELNKL
LGRVTIAQGGVLPNIQAVLL
PKKTESHHKAKGK
entity_2: GPGMPEPAKSAPAPKKGSKK
AVTKAQKKDGKKRKRSRKES
YSIYVYKVLKQVHPDTGISS
KAMGIMNSFVNDIFERIAGE
ASRLAHYNKRSTITSREIQT
AVRLLLPGELAKHAVSEGTK
AVTKYTSAK
- H_exch_rates
- assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2
- assigned_chemical_shifts_3
- assigned_chemical_shifts_4
- assigned_chemical_shifts_5
- assigned_chemical_shifts_6
- assigned_chemical_shifts_7
- assigned_chemical_shifts_8
- assigned_chemical_shifts_9
- assigned_chemical_shifts_10
- assigned_chemical_shifts_11
- assigned_chemical_shifts_12
- assigned_chemical_shifts_13
- assigned_chemical_shifts_14
- assigned_chemical_shifts_15
- assigned_chemical_shifts_16
- assigned_chemical_shifts_17
- assigned_chemical_shifts_18
- assigned_chemical_shifts_19
- assigned_chemical_shifts_20
- assigned_chemical_shifts_21
- assigned_chemical_shifts_22
- assigned_chemical_shifts_23
- assigned_chemical_shifts_24
- assigned_chemical_shifts_25
- assigned_chemical_shifts_26
- assigned_chemical_shifts_27
- assigned_chemical_shifts_28
- assigned_chemical_shifts_29
- assigned_chemical_shifts_30
- assigned_chemical_shifts_31
- assigned_chemical_shifts_32
- assigned_chemical_shifts_33
- assigned_chemical_shifts_34
- assigned_chemical_shifts_35
- assigned_chemical_shifts_36
- assigned_chemical_shifts_37
- assigned_chemical_shifts_38
- assigned_chemical_shifts_39
- assigned_chemical_shifts_40
- assigned_chemical_shifts_41
- assigned_chemical_shifts_42
- assigned_chemical_shifts_43
- assigned_chemical_shifts_44
- assigned_chemical_shifts_45
- assigned_chemical_shifts_46
- assigned_chemical_shifts_47
- assigned_chemical_shifts_48
- assigned_chemical_shifts_49
- assigned_chemical_shifts_50
- assigned_chemical_shifts_51
- assigned_chemical_shifts_52
- assigned_chemical_shifts_53
- assigned_chemical_shifts_54
- assigned_chemical_shifts_55
- assigned_chemical_shifts_56
- assigned_chemical_shifts_57
- assigned_chemical_shifts_58
- assigned_chemical_shifts_59
- assigned_chemical_shifts_60
- assigned_chemical_shifts_61
- heteronucl_NOEs
- heteronucl_NOEs_1
- heteronucl_NOEs_2
- heteronucl_NOEs_3
- heteronucl_NOEs_4
- heteronucl_NOEs_5
- heteronucl_NOEs_6
- heteronucl_NOEs_7
- heteronucl_NOEs_8
- heteronucl_NOEs_9
- heteronucl_NOEs_10
- heteronucl_NOEs_11
- heteronucl_NOEs_12
- heteronucl_NOEs_13
- heteronucl_NOEs_14
- heteronucl_NOEs_15
- heteronucl_NOEs_16
- heteronucl_NOEs_17
- heteronucl_NOEs_18
- heteronucl_NOEs_19
- heteronucl_NOEs_20
- heteronucl_NOEs_21
- heteronucl_NOEs_22
- heteronucl_NOEs_23
- heteronucl_NOEs_24
- heteronucl_NOEs_25
- heteronucl_NOEs_26
- heteronucl_NOEs_27
- heteronucl_NOEs_28
- heteronucl_T1_relaxation
- heteronucl_T1_relaxation_1
- heteronucl_T1_relaxation_2
- heteronucl_T1_relaxation_3
- heteronucl_T1_relaxation_4
- heteronucl_T1_relaxation_5
- heteronucl_T1_relaxation_6
- heteronucl_T1_relaxation_7
- heteronucl_T1_relaxation_8
- heteronucl_T1_relaxation_9
- heteronucl_T1_relaxation_10
- heteronucl_T1_relaxation_11
- heteronucl_T1_relaxation_12
- heteronucl_T1_relaxation_13
- heteronucl_T1_relaxation_14
- heteronucl_T1_relaxation_15
- heteronucl_T1_relaxation_16
- heteronucl_T1_relaxation_17
- heteronucl_T1_relaxation_18
- heteronucl_T1_relaxation_19
- heteronucl_T1_relaxation_20
- heteronucl_T1_relaxation_21
- heteronucl_T1_relaxation_22
- heteronucl_T1_relaxation_23
- heteronucl_T1_relaxation_24
- heteronucl_T1_relaxation_25
- heteronucl_T1_relaxation_26
- heteronucl_T1_relaxation_27
- heteronucl_T1_relaxation_28
- heteronucl_T2_relaxation
- heteronucl_T2_relaxation_1
- heteronucl_T2_relaxation_2
- heteronucl_T2_relaxation_3
- heteronucl_T2_relaxation_4
- heteronucl_T2_relaxation_5
- heteronucl_T2_relaxation_6
- heteronucl_T2_relaxation_7
- heteronucl_T2_relaxation_8
- heteronucl_T2_relaxation_9
- heteronucl_T2_relaxation_10
- heteronucl_T2_relaxation_11
- heteronucl_T2_relaxation_12
- heteronucl_T2_relaxation_13
- heteronucl_T2_relaxation_14
- heteronucl_T2_relaxation_15
- heteronucl_T2_relaxation_16
- heteronucl_T2_relaxation_17
- heteronucl_T2_relaxation_18
- heteronucl_T2_relaxation_19
- heteronucl_T2_relaxation_20
- heteronucl_T2_relaxation_21
- heteronucl_T2_relaxation_22
- heteronucl_T2_relaxation_23
- heteronucl_T2_relaxation_24
- heteronucl_T2_relaxation_25
- heteronucl_T2_relaxation_26
- heteronucl_T2_relaxation_27
- heteronucl_T2_relaxation_28
Data type | Count |
13C chemical shifts | 144 |
15N chemical shifts | 894 |
1H chemical shifts | 894 |
H exchange rates | 196 |
T1 relaxation values | 420 |
T2 relaxation values | 422 |
heteronuclear NOE values | 419 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | H2A | 1 |
2 | H2B | 2 |
Entities:
Entity 1, H2A 133 residues - Formula weight is not available
1 | GLY | PRO | GLY | MET | SER | GLY | ARG | GLY | LYS | GLN | ||||
2 | GLY | GLY | LYS | ALA | ARG | ALA | LYS | ALA | LYS | THR | ||||
3 | ARG | SER | SER | ARG | ALA | GLY | LEU | GLN | PHE | PRO | ||||
4 | VAL | GLY | ARG | VAL | HIS | ARG | LEU | LEU | ARG | LYS | ||||
5 | GLY | ASN | TYR | SER | GLU | ARG | VAL | GLY | ALA | GLY | ||||
6 | ALA | PRO | VAL | TYR | LEU | ALA | ALA | VAL | LEU | GLU | ||||
7 | TYR | LEU | THR | ALA | GLU | ILE | LEU | GLU | LEU | ALA | ||||
8 | GLY | ASN | ALA | ALA | ARG | ASP | ASN | LYS | LYS | THR | ||||
9 | ARG | ILE | ILE | PRO | ARG | HIS | LEU | GLN | LEU | ALA | ||||
10 | ILE | ARG | ASN | ASP | GLU | GLU | LEU | ASN | LYS | LEU | ||||
11 | LEU | GLY | ARG | VAL | THR | ILE | ALA | GLN | GLY | GLY | ||||
12 | VAL | LEU | PRO | ASN | ILE | GLN | ALA | VAL | LEU | LEU | ||||
13 | PRO | LYS | LYS | THR | GLU | SER | HIS | HIS | LYS | ALA | ||||
14 | LYS | GLY | LYS |
Entity 2, H2B 129 residues - Formula weight is not available
1 | GLY | PRO | GLY | MET | PRO | GLU | PRO | ALA | LYS | SER | ||||
2 | ALA | PRO | ALA | PRO | LYS | LYS | GLY | SER | LYS | LYS | ||||
3 | ALA | VAL | THR | LYS | ALA | GLN | LYS | LYS | ASP | GLY | ||||
4 | LYS | LYS | ARG | LYS | ARG | SER | ARG | LYS | GLU | SER | ||||
5 | TYR | SER | ILE | TYR | VAL | TYR | LYS | VAL | LEU | LYS | ||||
6 | GLN | VAL | HIS | PRO | ASP | THR | GLY | ILE | SER | SER | ||||
7 | LYS | ALA | MET | GLY | ILE | MET | ASN | SER | PHE | VAL | ||||
8 | ASN | ASP | ILE | PHE | GLU | ARG | ILE | ALA | GLY | GLU | ||||
9 | ALA | SER | ARG | LEU | ALA | HIS | TYR | ASN | LYS | ARG | ||||
10 | SER | THR | ILE | THR | SER | ARG | GLU | ILE | GLN | THR | ||||
11 | ALA | VAL | ARG | LEU | LEU | LEU | PRO | GLY | GLU | LEU | ||||
12 | ALA | LYS | HIS | ALA | VAL | SER | GLU | GLY | THR | LYS | ||||
13 | ALA | VAL | THR | LYS | TYR | THR | SER | ALA | LYS |
Samples:
sample_1: H2A, [U-100% 13C; U-100% 15N; U-80% 2H], 0.16 mM; H2B, [U-100% 13C; U-100% 15N; U-80% 2H], 0.16 mM; H3 0.16 mM; H4 0.16 mM; 145 bp DNA 0.08 mM
sample_2: H2A, [U-100% 13C; U-100% 15N; U-80% 2H], 0.2 mM; H2B, [U-100% 13C; U-100% 15N; U-80% 2H], 0.2 mM; H3 0.2 mM; H4 0.2 mM; 193 bp DNA 0.1 mM
sample_conditions_1: ionic strength: 0.4 M; pH: 6; pressure: 1 atm; temperature: 293 K
sample_conditions_2: ionic strength: 0.2 M; pH: 6; pressure: 1 atm; temperature: 293 K
sample_conditions_3: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 293 K
sample_conditions_4: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 293 K
sample_conditions_5: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 278 K
sample_conditions_6: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 283 K
sample_conditions_7: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 288 K
sample_conditions_8: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 298 K
sample_conditions_9: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 303 K
sample_conditions_10: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 308 K
sample_conditions_11: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 313 K
sample_conditions_12: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 318 K
sample_conditions_13: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 323 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_2 |
2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_3 |
2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_4 |
2D 1H-15N TROSY | sample_2 | isotropic | sample_conditions_4 |
2D 1H-15N TROSY | sample_2 | isotropic | sample_conditions_1 |
2D 1H-15N TROSY | sample_2 | isotropic | sample_conditions_5 |
2D 1H-15N | sample_2 | isotropic | sample_conditions_6 |
2D 1H-15N | sample_2 | isotropic | sample_conditions_7 |
2D 1H-15N | sample_2 | isotropic | sample_conditions_4 |
2D 1H-15N | sample_2 | isotropic | sample_conditions_8 |
2D 1H-15N | sample_2 | isotropic | sample_conditions_9 |
2D 1H-15N | sample_2 | isotropic | sample_conditions_10 |
2D 1H-15N | sample_2 | isotropic | sample_conditions_11 |
2D 1H-15N | sample_2 | isotropic | sample_conditions_12 |
2D 1H-15N | sample_2 | isotropic | sample_conditions_13 |
T1/R1 relaxation | sample_2 | isotropic | sample_conditions_4 |
T1/R1 relaxation | sample_2 | isotropic | sample_conditions_4 |
T1/R1 relaxation | sample_2 | isotropic | sample_conditions_1 |
T1/R1 relaxation | sample_2 | isotropic | sample_conditions_1 |
T1/R1 relaxation | sample_1 | isotropic | sample_conditions_4 |
T1/R1 relaxation | sample_1 | isotropic | sample_conditions_4 |
T1/R1 relaxation | sample_1 | isotropic | sample_conditions_1 |
T1/R1 relaxation | sample_1 | isotropic | sample_conditions_1 |
T2/R2 relaxation | sample_2 | isotropic | sample_conditions_4 |
T2/R2 relaxation | sample_2 | isotropic | sample_conditions_4 |
T2/R2 relaxation | sample_2 | isotropic | sample_conditions_1 |
T2/R2 relaxation | sample_2 | isotropic | sample_conditions_1 |
T2/R2 relaxation | sample_1 | isotropic | sample_conditions_4 |
T2/R2 relaxation | sample_1 | isotropic | sample_conditions_4 |
T2/R2 relaxation | sample_1 | isotropic | sample_conditions_1 |
T2/R2 relaxation | sample_1 | isotropic | sample_conditions_1 |
1H-15N heteronoe | sample_2 | isotropic | sample_conditions_4 |
1H-15N heteronoe | sample_2 | isotropic | sample_conditions_4 |
1H-15N heteronoe | sample_2 | isotropic | sample_conditions_1 |
1H-15N heteronoe | sample_2 | isotropic | sample_conditions_1 |
1H-15N heteronoe | sample_1 | isotropic | sample_conditions_4 |
1H-15N heteronoe | sample_1 | isotropic | sample_conditions_4 |
1H-15N heteronoe | sample_1 | isotropic | sample_conditions_1 |
1H-15N heteronoe | sample_1 | isotropic | sample_conditions_1 |
HETex-BEST-TROSY | sample_2 | isotropic | sample_conditions_4 |
HETex-BEST-TROSY | sample_2 | isotropic | sample_conditions_1 |
HETex-BEST-TROSY | sample_1 | isotropic | sample_conditions_4 |
HETex-BEST-TROSY | sample_1 | isotropic | sample_conditions_1 |
Software:
Olivia - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 950 MHz
- Bruker AVANCE III 600 MHz
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts