BMRB Entry 50444
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50444
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Title: Amide assignments of human GMCSF at low pH PubMed: 32897051
Deposition date: 2020-08-25 Original release date: 2021-04-16
Authors: Cui, Jennifer; Lisi, George
Citation: Cui, Jennifer; Zhang, Fuming; Nierzwicki, Lukasz; Palermo, Giulia; Linhardt, Robert; Lisi, George. "Mapping the Structural and Dynamic Determinants of pH-Sensitive Heparin Binding to Granulocyte Macrophage Colony Stimulating Factor" Biochemistry 59, 3541-3553 (2020).
Assembly members:
entity_1, polymer, 125 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b
Entity Sequences (FASTA):
entity_1: ARSPSPSTQPWEHVNAIQEA
RRLLNLSRDTAAEMNETVEV
ISEMFDLQEPTCLQTRLELY
KQGLRGSLTKLKGPLTMMAS
HYKQHCPPTPETSCATQIIT
FESFKENLKDFLLVIPFDCW
EPVQE
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 97 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GMCSF | 1 |
Entities:
Entity 1, GMCSF 125 residues - Formula weight is not available
| 1 | ALA | ARG | SER | PRO | SER | PRO | SER | THR | GLN | PRO | ||||
| 2 | TRP | GLU | HIS | VAL | ASN | ALA | ILE | GLN | GLU | ALA | ||||
| 3 | ARG | ARG | LEU | LEU | ASN | LEU | SER | ARG | ASP | THR | ||||
| 4 | ALA | ALA | GLU | MET | ASN | GLU | THR | VAL | GLU | VAL | ||||
| 5 | ILE | SER | GLU | MET | PHE | ASP | LEU | GLN | GLU | PRO | ||||
| 6 | THR | CYS | LEU | GLN | THR | ARG | LEU | GLU | LEU | TYR | ||||
| 7 | LYS | GLN | GLY | LEU | ARG | GLY | SER | LEU | THR | LYS | ||||
| 8 | LEU | LYS | GLY | PRO | LEU | THR | MET | MET | ALA | SER | ||||
| 9 | HIS | TYR | LYS | GLN | HIS | CYS | PRO | PRO | THR | PRO | ||||
| 10 | GLU | THR | SER | CYS | ALA | THR | GLN | ILE | ILE | THR | ||||
| 11 | PHE | GLU | SER | PHE | LYS | GLU | ASN | LEU | LYS | ASP | ||||
| 12 | PHE | LEU | LEU | VAL | ILE | PRO | PHE | ASP | CYS | TRP | ||||
| 13 | GLU | PRO | VAL | GLN | GLU |
Samples:
sample_1: GMCSF, [U-100% 15N], 300 uM; HEPES 20 mM; EDTA 1 mM
sample_conditions_1: ionic strength: 0.02 M; pH: 5.5; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts