BMRB Entry 50449

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50449

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Title:
Backbone Chemical Shift Assignments, Chemical shift perturbation and PRE data for XcpH soluble domain from P. aeruginosa
Deposition date:
2020-08-31
Original release date:
2021-05-03
Authors:
Escobar, Cristian; Forest, Katrina
Citation:

Citation: Escobar, Cristian. "Backbone Chemical Shift Assignments, Chemical shift perturbation and PRE data for XcpH soluble domain from P. aeruginosa"  Structure ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETG-20A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSTGFASTSRELDSEAERLA GLIGVLTDEAVLDNREYGLR LERDAYQVLRYDEAKARWLP VARDSHRLPEWAELTFELDG QPLVLAGSKGEKEQKKGTDQ PQLLILSSGELSPFRLRLAE RGPEGRALSLSSDGFRLPRV EVARR

Data sets:
Data typeCount
13C chemical shifts343
15N chemical shifts110
1H chemical shifts109
molecule interaction chemical shift values71

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1XcpH1

Entities:

Entity 1, XcpH 145 residues - Formula weight is not available

First Glycine residue is left after TEV cleavage and is not present in native protein. Amino acid numbering corresponds to the mature protein where the N-terminal phenylalanine left after signal peptide cleavage correspond to Phe1.

1   GLYSERTHRGLYPHEALASERTHRSERARG
2   GLULEUASPSERGLUALAGLUARGLEUALA
3   GLYLEUILEGLYVALLEUTHRASPGLUALA
4   VALLEUASPASNARGGLUTYRGLYLEUARG
5   LEUGLUARGASPALATYRGLNVALLEUARG
6   TYRASPGLUALALYSALAARGTRPLEUPRO
7   VALALAARGASPSERHISARGLEUPROGLU
8   TRPALAGLULEUTHRPHEGLULEUASPGLY
9   GLNPROLEUVALLEUALAGLYSERLYSGLY
10   GLULYSGLUGLNLYSLYSGLYTHRASPGLN
11   PROGLNLEULEUILELEUSERSERGLYGLU
12   LEUSERPROPHEARGLEUARGLEUALAGLU
13   ARGGLYPROGLUGLYARGALALEUSERLEU
14   SERSERASPGLYPHEARGLEUPROARGVAL
15   GLUVALALAARGARG

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks