BMRB Entry 50452

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50452

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Title:
DegP-PDZ1PDZ2-25C
Deposition date:
2020-09-02
Original release date:
2021-10-15
Authors:
Sulskis, Darius; Thoma, Johannes; Burmann, Bjorn
Citation:

Citation: Sulskis, Darius; Thoma, Johannes; Burmann, Bjorn. "Structural basis of DegP protease temperature-dependent activation"  Sci. Adv. 7, eabj1816-eabj1816 (2021).
PubMed: 34878848

Assembly members:

Assembly members:
entity_1, polymer, 188 residues, 19610 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KRGELGIMGTELNSELAKAM KVDAQRGAFVSQVLPNSSAA KAGIKAGDVITSLNGKPISS FAALRAQVGTMPVGSKLTLG LLRDGKQVNVNLELQQSSQN QVDSSSIFNGIEGAEMSNKG KDQGVVVNNVKTGTPAAQIG LKKGDVIIGANQQAVKNIAE LRKVLDSKPSVLALNIQRGD STIYLLMQ

Data sets:
Data typeCount
13C chemical shifts372
15N chemical shifts135
1H chemical shifts135

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ1-PDZ21

Entities:

Entity 1, PDZ1-PDZ2 188 residues - 19610 Da.

PDZ1-PDZ2 domain (261-448 a.a.) of DegP

1   LYSARGGLYGLULEUGLYILEMETGLYTHR
2   GLULEUASNSERGLULEUALALYSALAMET
3   LYSVALASPALAGLNARGGLYALAPHEVAL
4   SERGLNVALLEUPROASNSERSERALAALA
5   LYSALAGLYILELYSALAGLYASPVALILE
6   THRSERLEUASNGLYLYSPROILESERSER
7   PHEALAALALEUARGALAGLNVALGLYTHR
8   METPROVALGLYSERLYSLEUTHRLEUGLY
9   LEULEUARGASPGLYLYSGLNVALASNVAL
10   ASNLEUGLULEUGLNGLNSERSERGLNASN
11   GLNVALASPSERSERSERILEPHEASNGLY
12   ILEGLUGLYALAGLUMETSERASNLYSGLY
13   LYSASPGLNGLYVALVALVALASNASNVAL
14   LYSTHRGLYTHRPROALAALAGLNILEGLY
15   LEULYSLYSGLYASPVALILEILEGLYALA
16   ASNGLNGLNALAVALLYSASNILEALAGLU
17   LEUARGLYSVALLEUASPSERLYSPROSER
18   VALLEUALALEUASNILEGLNARGGLYASP
19   SERTHRILETYRLEULEUMETGLN

Related Database Links:

UNP P0C0V0
AlphaFold P15724

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks