BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50458

Title: Backbone amide group 15N and 1H assignments and 15N relaxation data for human PARP-1 CAT domain complexed to EB-47   PubMed: 33511412

Deposition date: 2020-09-03 Original release date: 2020-09-15

Authors: Ogden, Tom; Yang, Ji-Chun; Neuhaus, David

Citation: Ogden, Tom; Yang, Ji-Chun; Schimpl, Marianne; Easton, Laura; Underwood, Elizabeth; Rawlins, Philip; McCauley, Michael; Langelier, Marie-France; Pascal, John; Embrey, Kevin; Neuhaus, David. "Dynamics of the HD regulatory subdomain of PARP-1; substrate access and allostery in PARP activation and inhibition"  Nucleic Acids Res. 49, 2266-2288 (2021).

Assembly members:
entity_1, polymer, 360 residues, Formula weight is not available
entity_UHB, non-polymer, 537.528 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):
entity_1: GTVNPGTKSKLPKPVQDLIK MIFDVESMKKAMVEYEIDLQ KMPLGKLSKRQIQAAYSILS EVQQAVSQGSSDSQILDLSN RFYTLIPHDFGMKKPPLLNN ADSVQAKAEMLDNLLDIEVA YSLLRGGSDDSSKDPIDVNY EKLKTDIKVVDRDSEEAEII RKYVKNTHATTHNAYDLEVI DIFKIEREGECQRYKPFKQL HNRRLLWHGSRTTNFAGILS QGLRIAPPEAPVTGYMFGKG IYFADMVSKSANYCHTSQGD PIGLILLGEVALGNMYELKH ASHISKLPKGKHSVKGLGKT TPDPSANISLDGVDVPLGTG ISSGVNDTSLLYNEYIVYDI AQVNLKYLLKLKFNFKTSLW

Data sets:
Data typeCount
15N chemical shifts301
1H chemical shifts301
T1 relaxation values238
T1rho relaxation values235
heteronuclear NOE values295

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PARP-1_CAT_domain1
2EB-472

Entities:

Entity 1, PARP-1_CAT_domain 360 residues - Formula weight is not available

The first residue in the sequence supplied here is a non-native glycine arising from cloning. The remaining residues are residues 656-1014 of human PARP-1 (the isoform having Ala at position 762).

1   GLYTHRVALASNPROGLYTHRLYSSERLYS
2   LEUPROLYSPROVALGLNASPLEUILELYS
3   METILEPHEASPVALGLUSERMETLYSLYS
4   ALAMETVALGLUTYRGLUILEASPLEUGLN
5   LYSMETPROLEUGLYLYSLEUSERLYSARG
6   GLNILEGLNALAALATYRSERILELEUSER
7   GLUVALGLNGLNALAVALSERGLNGLYSER
8   SERASPSERGLNILELEUASPLEUSERASN
9   ARGPHETYRTHRLEUILEPROHISASPPHE
10   GLYMETLYSLYSPROPROLEULEUASNASN
11   ALAASPSERVALGLNALALYSALAGLUMET
12   LEUASPASNLEULEUASPILEGLUVALALA
13   TYRSERLEULEUARGGLYGLYSERASPASP
14   SERSERLYSASPPROILEASPVALASNTYR
15   GLULYSLEULYSTHRASPILELYSVALVAL
16   ASPARGASPSERGLUGLUALAGLUILEILE
17   ARGLYSTYRVALLYSASNTHRHISALATHR
18   THRHISASNALATYRASPLEUGLUVALILE
19   ASPILEPHELYSILEGLUARGGLUGLYGLU
20   CYSGLNARGTYRLYSPROPHELYSGLNLEU
21   HISASNARGARGLEULEUTRPHISGLYSER
22   ARGTHRTHRASNPHEALAGLYILELEUSER
23   GLNGLYLEUARGILEALAPROPROGLUALA
24   PROVALTHRGLYTYRMETPHEGLYLYSGLY
25   ILETYRPHEALAASPMETVALSERLYSSER
26   ALAASNTYRCYSHISTHRSERGLNGLYASP
27   PROILEGLYLEUILELEULEUGLYGLUVAL
28   ALALEUGLYASNMETTYRGLULEULYSHIS
29   ALASERHISILESERLYSLEUPROLYSGLY
30   LYSHISSERVALLYSGLYLEUGLYLYSTHR
31   THRPROASPPROSERALAASNILESERLEU
32   ASPGLYVALASPVALPROLEUGLYTHRGLY
33   ILESERSERGLYVALASNASPTHRSERLEU
34   LEUTYRASNGLUTYRILEVALTYRASPILE
35   ALAGLNVALASNLEULYSTYRLEULEULYS
36   LEULYSPHEASNPHELYSTHRSERLEUTRP

Entity 2, EB-47 - C24 H27 N9 O6 - 537.528 Da.

1   UHB

Samples:

sample_1: human PARP-1 CAT domain, [U-15N], 0.4 mM; TRIS, [U-2H], 50 mM; sodium chloride 50 mM; DTT, [U-2H], 2 mM; EB-47 0.2 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D 15N-separated NOESYsample_1isotropicsample_conditions_1
2D 1H-15N TROSY-based steady-state 15N{1H} NOE measurementsample_1isotropicsample_conditions_1
2D 1H-15N TROSY-based 15N T1 measurementsample_1isotropicsample_conditions_1
2D 1H-15N TROSY-based 15N T1rho measurementsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2 and 3.5 - processing

MddNMR v2.4 - processing

NMRPipe v8.7 - processing

ANALYSIS v2.4.2 - data analysis

SPARKY v3.115 - data analysis

NMR spectrometers:

  • Bruker AVANCE III HD 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts