BMRB Entry 50470
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50470
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignment for Human Atg3 without N-terminal 25 resides PubMed: 33446636
Deposition date: 2020-09-17 Original release date: 2020-11-25
Authors: Ye, Yansheng; Tyndall, Erin; Tian, Fang
Citation: Ye, Yansheng; Tyndall, Erin; Bui, Van; Tang, Zhenyuan; Shen, Yan; Jiang, Xuejun; Flanagan, John; Wang, Hong-Gang; Tian, Fang. "An N-terminal conserved region in human Atg3 couples membrane curvature sensitivity to conjugase activity during autophagy" Nat. Commun. 12, 374-374 (2021).
Assembly members:
entity_1, polymer, 289 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: SKFKETGVITPEEFVAAGDH
LVHHCPTWQWATGEELKVKA
YLPTGKQFLVTKNVPCYKRC
KQMEYSDELEAIIEEDDGDG
GWVDTYHNTGITGITEAVKE
ITLENKDNIRLQDCSALCEE
EEDEDEGEAADMEEYEESGL
LETDEATLDTRKIVEACKAK
TDAGGEDAILQTRTYDLYIT
YDKYYQTPRLWLFGYDEQRQ
PLTVEHMYEDISQDHVKKTV
TIENHPHLPPPPMCSVHPCR
HAEVMKKIIETVAEGGGELG
VHMYLLIFLKFVQAVIPTIE
YDYTRHFTM
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 727 |
| 15N chemical shifts | 220 |
| 1H chemical shifts | 220 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Atg3 | 1 |
Entities:
Entity 1, Atg3 289 residues - Formula weight is not available
| 1 | SER | LYS | PHE | LYS | GLU | THR | GLY | VAL | ILE | THR | ||||
| 2 | PRO | GLU | GLU | PHE | VAL | ALA | ALA | GLY | ASP | HIS | ||||
| 3 | LEU | VAL | HIS | HIS | CYS | PRO | THR | TRP | GLN | TRP | ||||
| 4 | ALA | THR | GLY | GLU | GLU | LEU | LYS | VAL | LYS | ALA | ||||
| 5 | TYR | LEU | PRO | THR | GLY | LYS | GLN | PHE | LEU | VAL | ||||
| 6 | THR | LYS | ASN | VAL | PRO | CYS | TYR | LYS | ARG | CYS | ||||
| 7 | LYS | GLN | MET | GLU | TYR | SER | ASP | GLU | LEU | GLU | ||||
| 8 | ALA | ILE | ILE | GLU | GLU | ASP | ASP | GLY | ASP | GLY | ||||
| 9 | GLY | TRP | VAL | ASP | THR | TYR | HIS | ASN | THR | GLY | ||||
| 10 | ILE | THR | GLY | ILE | THR | GLU | ALA | VAL | LYS | GLU | ||||
| 11 | ILE | THR | LEU | GLU | ASN | LYS | ASP | ASN | ILE | ARG | ||||
| 12 | LEU | GLN | ASP | CYS | SER | ALA | LEU | CYS | GLU | GLU | ||||
| 13 | GLU | GLU | ASP | GLU | ASP | GLU | GLY | GLU | ALA | ALA | ||||
| 14 | ASP | MET | GLU | GLU | TYR | GLU | GLU | SER | GLY | LEU | ||||
| 15 | LEU | GLU | THR | ASP | GLU | ALA | THR | LEU | ASP | THR | ||||
| 16 | ARG | LYS | ILE | VAL | GLU | ALA | CYS | LYS | ALA | LYS | ||||
| 17 | THR | ASP | ALA | GLY | GLY | GLU | ASP | ALA | ILE | LEU | ||||
| 18 | GLN | THR | ARG | THR | TYR | ASP | LEU | TYR | ILE | THR | ||||
| 19 | TYR | ASP | LYS | TYR | TYR | GLN | THR | PRO | ARG | LEU | ||||
| 20 | TRP | LEU | PHE | GLY | TYR | ASP | GLU | GLN | ARG | GLN | ||||
| 21 | PRO | LEU | THR | VAL | GLU | HIS | MET | TYR | GLU | ASP | ||||
| 22 | ILE | SER | GLN | ASP | HIS | VAL | LYS | LYS | THR | VAL | ||||
| 23 | THR | ILE | GLU | ASN | HIS | PRO | HIS | LEU | PRO | PRO | ||||
| 24 | PRO | PRO | MET | CYS | SER | VAL | HIS | PRO | CYS | ARG | ||||
| 25 | HIS | ALA | GLU | VAL | MET | LYS | LYS | ILE | ILE | GLU | ||||
| 26 | THR | VAL | ALA | GLU | GLY | GLY | GLY | GLU | LEU | GLY | ||||
| 27 | VAL | HIS | MET | TYR | LEU | LEU | ILE | PHE | LEU | LYS | ||||
| 28 | PHE | VAL | GLN | ALA | VAL | ILE | PRO | THR | ILE | GLU | ||||
| 29 | TYR | ASP | TYR | THR | ARG | HIS | PHE | THR | MET |
Samples:
sample_1: Human Atg3 without N-terminal 25 residues, [U-13C; U-15N], 0.4 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
NMRView - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts