BMRB Entry 50473
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50473
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Title: Backbone Resonance Assignments of OmpR DNA-binding domain. PubMed: 33152421
Deposition date: 2020-09-21 Original release date: 2020-12-29
Authors: Sadotra, Sushant; Lou, Yuan-Chao; Chen, Chinpan
Citation: Sadotra, Sushant; Lou, Yuan-Chao; Tang, Hao-Cheng; Chiu, Yi-Chih; Hsu, Chun-Hua; Chen, Chinpan. "Structural basis for promoter DNA recognition by the response regulator OmpR" J. Struct. Biol. 213, 107638-107638 (2020).
Assembly members:
entity_1, polymer, 103 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-29b
Entity Sequences (FASTA):
entity_1: VIAFGKFKLNLGTREMFRED
EPMPLTSGEFAVLKALVSHP
REPLSRDKLMNLARGREYSA
MERSIDVQISRLRRMVEEDP
AHPRYIQTVWGLGYVFVPDG
SKA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 262 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 92 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | OmpRc | 1 |
Entities:
Entity 1, OmpRc 103 residues - Formula weight is not available
| 1 | VAL | ILE | ALA | PHE | GLY | LYS | PHE | LYS | LEU | ASN | ||||
| 2 | LEU | GLY | THR | ARG | GLU | MET | PHE | ARG | GLU | ASP | ||||
| 3 | GLU | PRO | MET | PRO | LEU | THR | SER | GLY | GLU | PHE | ||||
| 4 | ALA | VAL | LEU | LYS | ALA | LEU | VAL | SER | HIS | PRO | ||||
| 5 | ARG | GLU | PRO | LEU | SER | ARG | ASP | LYS | LEU | MET | ||||
| 6 | ASN | LEU | ALA | ARG | GLY | ARG | GLU | TYR | SER | ALA | ||||
| 7 | MET | GLU | ARG | SER | ILE | ASP | VAL | GLN | ILE | SER | ||||
| 8 | ARG | LEU | ARG | ARG | MET | VAL | GLU | GLU | ASP | PRO | ||||
| 9 | ALA | HIS | PRO | ARG | TYR | ILE | GLN | THR | VAL | TRP | ||||
| 10 | GLY | LEU | GLY | TYR | VAL | PHE | VAL | PRO | ASP | GLY | ||||
| 11 | SER | LYS | ALA |
Samples:
sample_1: OmpRc, [U-100% 15N], 1.1 mM
sample_conditions_1: pH: 6; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts