BMRB Entry 50478

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50478

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Human growth hormone receptor intracellular domain
Deposition date:
2020-09-23
Original release date:
2020-10-23
Authors:
Kragelund, Birthe; Nikolajsen, Louise; Haxholm, Gitte; Seiffert, Pernille
Citation:

Citation: Haxholm, Gitte; Nikolajsen, Louise; Olsen, Johan; Fredsted, Jacob; Larsen, Flemming; Goffin, Vincent; Pedersen, Stine; Brooks, Andrew; Waters, Michael; Kragelund, Birthe. "Intrinsically disordered cytoplasmic domains of two cytokine receptors mediate conserved interactions with membranes."  Biochem. J. 468, 495-506 (2015).
PubMed: 25846210

Assembly members:

Assembly members:
entity_1, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSSKQQRIKMLILPPVPVPK IKGIDPDLLKEGKLEEVNTI LAIHDSYKPEFHSDDSWVEF IELDIDEPDEKTEESDTDRL LSSDHEKSHSNLGVKDGDSG RTS

Data sets:
Data typeCount
13C chemical shifts289
15N chemical shifts93
1H chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GHR-ICD270-3701

Entities:

Entity 1, GHR-ICD270-370 103 residues - Formula weight is not available

Residues 270 - 370 of the long isoform of GHR includes two non-native residues as remnants from cloning (G268 and S269).

1   GLYSERSERLYSGLNGLNARGILELYSMET
2   LEUILELEUPROPROVALPROVALPROLYS
3   ILELYSGLYILEASPPROASPLEULEULYS
4   GLUGLYLYSLEUGLUGLUVALASNTHRILE
5   LEUALAILEHISASPSERTYRLYSPROGLU
6   PHEHISSERASPASPSERTRPVALGLUPHE
7   ILEGLULEUASPILEASPGLUPROASPGLU
8   LYSTHRGLUGLUSERASPTHRASPARGLEU
9   LEUSERSERASPHISGLULYSSERHISSER
10   ASNLEUGLYVALLYSASPGLYASPSERGLY
11   ARGTHRSER

Related Database Links:

UNP P10912-1
AlphaFold Q9HCX2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks