BMRB Entry 50478
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50478
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Title: Human growth hormone receptor intracellular domain PubMed: 25846210
Deposition date: 2020-09-23 Original release date: 2020-10-23
Authors: Kragelund, Birthe; Nikolajsen, Louise; Haxholm, Gitte; Seiffert, Pernille
Citation: Haxholm, Gitte; Nikolajsen, Louise; Olsen, Johan; Fredsted, Jacob; Larsen, Flemming; Goffin, Vincent; Pedersen, Stine; Brooks, Andrew; Waters, Michael; Kragelund, Birthe. "Intrinsically disordered cytoplasmic domains of two cytokine receptors mediate conserved interactions with membranes." Biochem. J. 468, 495-506 (2015).
Assembly members:
entity_1, polymer, 103 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P-1
Entity Sequences (FASTA):
entity_1: GSSKQQRIKMLILPPVPVPK
IKGIDPDLLKEGKLEEVNTI
LAIHDSYKPEFHSDDSWVEF
IELDIDEPDEKTEESDTDRL
LSSDHEKSHSNLGVKDGDSG
RTS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 289 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 93 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GHR-ICD270-370 | 1 |
Entities:
Entity 1, GHR-ICD270-370 103 residues - Formula weight is not available
Residues 270 - 370 of the long isoform of GHR includes two non-native residues as remnants from cloning (G268 and S269).
| 1 | GLY | SER | SER | LYS | GLN | GLN | ARG | ILE | LYS | MET | ||||
| 2 | LEU | ILE | LEU | PRO | PRO | VAL | PRO | VAL | PRO | LYS | ||||
| 3 | ILE | LYS | GLY | ILE | ASP | PRO | ASP | LEU | LEU | LYS | ||||
| 4 | GLU | GLY | LYS | LEU | GLU | GLU | VAL | ASN | THR | ILE | ||||
| 5 | LEU | ALA | ILE | HIS | ASP | SER | TYR | LYS | PRO | GLU | ||||
| 6 | PHE | HIS | SER | ASP | ASP | SER | TRP | VAL | GLU | PHE | ||||
| 7 | ILE | GLU | LEU | ASP | ILE | ASP | GLU | PRO | ASP | GLU | ||||
| 8 | LYS | THR | GLU | GLU | SER | ASP | THR | ASP | ARG | LEU | ||||
| 9 | LEU | SER | SER | ASP | HIS | GLU | LYS | SER | HIS | SER | ||||
| 10 | ASN | LEU | GLY | VAL | LYS | ASP | GLY | ASP | SER | GLY | ||||
| 11 | ARG | THR | SER |
Samples:
sample_1: Human growth hormone receptor intracellular domain 270-370, [U-95% 13C; U-95% 15N], 500 mM; Na2HPO4/NaH2PO4 20 mM; tris(2-carboxyethyl)phosphine (TCEP) 6.5 mM; 2,2-dimethyl-2-silapentane-5-sulfonic acid (DSS) 0.5 mM
sample_conditions_1: ionic strength: 20 mM; pH: 7.3; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - analyses
NMRPipe - transformation and visualization
NMR spectrometers:
- Agilent INOVA 750 MHz
- Agilent INOVA 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts