BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50482

Title: RelAdd_homodimer T1, T2 and NOE   PubMed: 33555182

Deposition date: 2020-09-28 Original release date: 2021-04-13

Authors: Kumar, Manish; Dhaka, Nitin; Mukherjee, Sulakshana

Citation: Kumar, Manish; Dhaka, Nitin; Raza, Tahseen; Dadhwal, Prikshat; Atreya, Hanudatta; Mukherjee, Sulakshana. "Domain stability regulated through the dimer interface controls the formation kinetics of a specific NF-kappaB dimer"  Biochemistry 60, 513-523 (2021).

Assembly members:
entity_1, polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):
entity_1: NTAELKICRVNRNSGSCLGG DEIFLLCDKVQKEDIEVYFT GPGWEARGSFSQADVHRQVA IVFRTPPYADPSLQAPVRVS MQLRRPSDRELSEPMEFQYL PD

Data sets:
Data typeCount
T1 relaxation values76
T2 relaxation values76
heteronuclear NOE values75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RelA, chain 11
2RelA, chain 21

Entities:

Entity 1, RelA, chain 1 102 residues - Formula weight is not available

1   ASNTHRALAGLULEULYSILECYSARGVAL
2   ASNARGASNSERGLYSERCYSLEUGLYGLY
3   ASPGLUILEPHELEULEUCYSASPLYSVAL
4   GLNLYSGLUASPILEGLUVALTYRPHETHR
5   GLYPROGLYTRPGLUALAARGGLYSERPHE
6   SERGLNALAASPVALHISARGGLNVALALA
7   ILEVALPHEARGTHRPROPROTYRALAASP
8   PROSERLEUGLNALAPROVALARGVALSER
9   METGLNLEUARGARGPROSERASPARGGLU
10   LEUSERGLUPROMETGLUPHEGLNTYRLEU
11   PROASP

Samples:

sample_1: RelA, [U-99% 15N], 440 uM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
T1/R1 relaxationsample_1isotropicsample_conditions_1
T2/R2 relaxationsample_1isotropicsample_conditions_1
1H-15N heteronoesample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

Relax - data analysis

CARA - data analysis

NMR spectrometers:

  • Bruker Ascend 800 MHz