BMRB Entry 50495
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50495
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Title: RelAdd_mutant PubMed: 33555182
Deposition date: 2020-10-01 Original release date: 2021-04-13
Authors: Kumar, Manish; Dhaka, Nitin; Raza, Tahseen; Atreya, Hanudatta; Mukherjee, Sulakshana
Citation: Kumar, Manish; Dhaka, Nitin; Raza, Tahseen; Dadhwal, Prikshat; Atreya, Hanudatta; Mukherjee, Sulakshana. "Domain stability regulated through the dimer interface controls the formation kinetics of a specific NF-kappaB dimer" Biochemistry 60, 513-523 (2021).
Assembly members:
entity_1, polymer, 102 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
entity_1: NTAELKICRVNRNSGSCLGG
DEIFLLCDKVQKEDIEVYFT
GPGWEARGSFSQADVHRQFA
IVFRTPPYADPSLQAPVRVS
MQLRRPSDRELSEPMEFQYL
PD
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| T1 relaxation values | 70 |
| T2 relaxation values | 70 |
| heteronuclear NOE values | 70 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RelA(V248F), chain 1 | 1 |
| 2 | RelA(V248F), chain 2 | 1 |
Entities:
Entity 1, RelA(V248F), chain 1 102 residues - Formula weight is not available
Starts at 190
| 1 | ASN | THR | ALA | GLU | LEU | LYS | ILE | CYS | ARG | VAL | ||||
| 2 | ASN | ARG | ASN | SER | GLY | SER | CYS | LEU | GLY | GLY | ||||
| 3 | ASP | GLU | ILE | PHE | LEU | LEU | CYS | ASP | LYS | VAL | ||||
| 4 | GLN | LYS | GLU | ASP | ILE | GLU | VAL | TYR | PHE | THR | ||||
| 5 | GLY | PRO | GLY | TRP | GLU | ALA | ARG | GLY | SER | PHE | ||||
| 6 | SER | GLN | ALA | ASP | VAL | HIS | ARG | GLN | PHE | ALA | ||||
| 7 | ILE | VAL | PHE | ARG | THR | PRO | PRO | TYR | ALA | ASP | ||||
| 8 | PRO | SER | LEU | GLN | ALA | PRO | VAL | ARG | VAL | SER | ||||
| 9 | MET | GLN | LEU | ARG | ARG | PRO | SER | ASP | ARG | GLU | ||||
| 10 | LEU | SER | GLU | PRO | MET | GLU | PHE | GLN | TYR | LEU | ||||
| 11 | PRO | ASP |
Samples:
sample_1: RelA(V248F) dd, [U-99% 15N], 0.44 mM; TRIS 20 mM; sodium chloride 50 mM; beta-mercaptoethanol 20 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| T1/R1 relaxation | sample_1 | isotropic | sample_conditions_1 |
| T2/R2 relaxation | sample_1 | isotropic | sample_conditions_1 |
| 1H-15N heteronoe | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
CARA - data analysis
Relax - data analysis
NMR spectrometers:
- Bruker Ascend 800 MHz