BMRB Entry 50512
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50512
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Title: Backbone chemical shifts assignments of PLEKHA7 PubMed: 33878292
Deposition date: 2020-10-14 Original release date: 2021-06-30
Authors: Yao, Yong; Yu, Jinghua; Marassi, Francesca
Citation: Aleshin, Alexander; Yao, Yong; Iftikhar, Amer; Bobkov, Andrey; Yu, Jinghua; Cadwell, Gregory; Klein, Michael; Dong, Chuqiao; Bankston, Laurie; Liddington, Robert; Im, Wonpil; Powis, Garth; Marassi, Francesca. "Structural basis for the association of PLEKHA7 with membrane-embedded phosphatidylinositol lipids." Structure 29, 1029-1039 (2021).
Assembly members:
entity_1, polymer, 127 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
Entity Sequences (FASTA):
entity_1: GPLGSPVVVRGWLHKQDSSG
MRLWKRRWFVLADYCLFYYK
DSREEAVLGSIPLPSYVISP
VAPEDRISRKYSFKAVHTGM
RALIYNSSTAGSQAEQSGMR
TYYFSADTQEDMNAWVRAMN
QAAQVLS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 227 |
| 15N chemical shifts | 114 |
| 1H chemical shifts | 114 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PLEKHA7 | 1 |
Entities:
Entity 1, PLEKHA7 127 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | PRO | VAL | VAL | VAL | ARG | ||||
| 2 | GLY | TRP | LEU | HIS | LYS | GLN | ASP | SER | SER | GLY | ||||
| 3 | MET | ARG | LEU | TRP | LYS | ARG | ARG | TRP | PHE | VAL | ||||
| 4 | LEU | ALA | ASP | TYR | CYS | LEU | PHE | TYR | TYR | LYS | ||||
| 5 | ASP | SER | ARG | GLU | GLU | ALA | VAL | LEU | GLY | SER | ||||
| 6 | ILE | PRO | LEU | PRO | SER | TYR | VAL | ILE | SER | PRO | ||||
| 7 | VAL | ALA | PRO | GLU | ASP | ARG | ILE | SER | ARG | LYS | ||||
| 8 | TYR | SER | PHE | LYS | ALA | VAL | HIS | THR | GLY | MET | ||||
| 9 | ARG | ALA | LEU | ILE | TYR | ASN | SER | SER | THR | ALA | ||||
| 10 | GLY | SER | GLN | ALA | GLU | GLN | SER | GLY | MET | ARG | ||||
| 11 | THR | TYR | TYR | PHE | SER | ALA | ASP | THR | GLN | GLU | ||||
| 12 | ASP | MET | ASN | ALA | TRP | VAL | ARG | ALA | MET | ASN | ||||
| 13 | GLN | ALA | ALA | GLN | VAL | LEU | SER |
Samples:
sample_1: PLEKHA7, [U-100% 13C; U-100% 15N], 0.1 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 6.0; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
NMRViewJ - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts