BMRB Entry 50512

Name: Backbone chemical shifts assignments of PLEKHA7
Published: 2021-06-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50512

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shifts assignments of PLEKHA7

Deposition date:

2020-10-14

Original release date:

2021-06-30

Authors:

Yao, Yong; Yu, Jinghua; Marassi, Francesca

Citation:

Citation: Aleshin, Alexander; Yao, Yong; Iftikhar, Amer; Bobkov, Andrey; Yu, Jinghua; Cadwell, Gregory; Klein, Michael; Dong, Chuqiao; Bankston, Laurie; Liddington, Robert; Im, Wonpil; Powis, Garth; Marassi, Francesca. "Structural basis for the association of PLEKHA7 with membrane-embedded phosphatidylinositol lipids." Structure 29, 1029-1039 (2021).
PubMed: 33878292

Assembly members:

Assembly members:
entity_1, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPLGSPVVVRGWLHKQDSSG MRLWKRRWFVLADYCLFYYK DSREEAVLGSIPLPSYVISP VAPEDRISRKYSFKAVHTGM RALIYNSSTAGSQAEQSGMR TYYFSADTQEDMNAWVRAMN QAAQVLS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	227
15N chemical shifts	114
1H chemical shifts	114

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PLEKHA7	1

Entities:

Entity 1, PLEKHA7 127 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

PRO

VAL

ARG

2

GLY

TRP

LEU

HIS

LYS

GLN

ASP

SER

GLY

3

MET

ARG

LEU

TRP

LYS

ARG

TRP

PHE

VAL

4

LEU

ALA

ASP

TYR

CYS

LEU

PHE

TYR

LYS

5

ASP

SER

ARG

GLU

ALA

VAL

LEU

GLY

SER

6

ILE

PRO

LEU

PRO

SER

TYR

VAL

ILE

SER

PRO

7

VAL

ALA

PRO

GLU

ASP

ARG

ILE

SER

ARG

LYS

8

TYR

SER

PHE

LYS

ALA

VAL

HIS

THR

GLY

MET

9

ARG

ALA

LEU

ILE

TYR

ASN

SER

THR

ALA

10

GLY

SER

GLN

ALA

GLU

GLN

SER

GLY

MET

ARG

11

THR

TYR

PHE

SER

ALA

ASP

THR

GLN

GLU

12

ASP

MET

ASN

ALA

TRP

VAL

ARG

ALA

MET

ASN

13

GLN

ALA

GLN

VAL

LEU

SER

Samples:

sample_1: PLEKHA7, [U-100% 13C; U-100% 15N], 0.1 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.0; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRViewJ - data analysis

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks