BMRB Entry 50523

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50523

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Title:
SsoSSB 1-114
Deposition date:
2020-10-20
Original release date:
2021-06-30
Authors:
Yang, Min June; Park, Chin-Ju; Lee, Woonghee
Citation:

Citation: Yang, Min June; Lee, Woonghee; Park, Chin-Ju. "Resonance assignments and secondary structure of thermophile single-stranded DNA binding protein from Sulfolobus solfataricus at 323K."  Biomol. NMR Assign. 15, 159-164 (2021).
PubMed: 33405014

Assembly members:

Assembly members:
entity_1, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Sulfolobus solfataricus   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Saccharolobus solfataricus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEEKVGNLKPNMESVNVTVR VLEASEARQIQTKNGVRTIS EAIVGDETGRVKLTLWGKHA GSIKEGQVVKIENAWTTAFK GQVQLNAGSKTKIAEASEDG FPESSQIPENTPTAENLYFQ SGSHHHHHH

Data sets:
Data typeCount
13C chemical shifts483
15N chemical shifts116
1H chemical shifts756

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1SsoSSB1

Entities:

Entity 1, SsoSSB 129 residues - Formula weight is not available

1   METGLUGLULYSVALGLYASNLEULYSPRO
2   ASNMETGLUSERVALASNVALTHRVALARG
3   VALLEUGLUALASERGLUALAARGGLNILE
4   GLNTHRLYSASNGLYVALARGTHRILESER
5   GLUALAILEVALGLYASPGLUTHRGLYARG
6   VALLYSLEUTHRLEUTRPGLYLYSHISALA
7   GLYSERILELYSGLUGLYGLNVALVALLYS
8   ILEGLUASNALATRPTHRTHRALAPHELYS
9   GLYGLNVALGLNLEUASNALAGLYSERLYS
10   THRLYSILEALAGLUALASERGLUASPGLY
11   PHEPROGLUSERSERGLNILEPROGLUASN
12   THRPROTHRALAGLUASNLEUTYRPHEGLN
13   SERGLYSERHISHISHISHISHISHIS

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