BMRB Entry 50524
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50524
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for c-FLIPs_C8-1a compex PubMed: 36084903
Deposition date: 2020-10-20 Original release date: 2022-10-14
Authors: Hu, Kaifeng; Bai, Zhiqiang
Citation: Hu, Kaifeng; Bai, Zhiqiang. "Expression, purification, and characterization of c-FLIP tandem death effector domains from Escherichia coli" Protein Expr. Purif. 200, 106168-106168 (2022).
Assembly members:
entity_1, polymer, 185 residues, Formula weight is not available
entity_2, polymer, 8 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a
Entity Sequences (FASTA):
entity_1: MGRSDSAEVIHQVEEALDTD
EKEMLLFLCRDVAIDVVPPN
VRDLLDILRERGKLSVGDLA
ELLYRVRRFDLLKRILKMDR
KAVETHLLRNPHLVSDYRVL
MAEIGEDLDKSDVSSLIGLM
KDYMGRGKISKEKSFLDLVV
ELEKLNLVAPDQLDLLEKCL
KNIHRIDLKTKIQKYKQLEH
HHHHH
entity_2: DFSRNLYD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 746 |
| 15N chemical shifts | 179 |
| 1H chemical shifts | 1259 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | c-FLIPs | 1 |
| 2 | C8-1a | 2 |
Entities:
Entity 1, c-FLIPs 185 residues - Formula weight is not available
| 1 | MET | GLY | ARG | SER | ASP | SER | ALA | GLU | VAL | ILE | ||||
| 2 | HIS | GLN | VAL | GLU | GLU | ALA | LEU | ASP | THR | ASP | ||||
| 3 | GLU | LYS | GLU | MET | LEU | LEU | PHE | LEU | CYS | ARG | ||||
| 4 | ASP | VAL | ALA | ILE | ASP | VAL | VAL | PRO | PRO | ASN | ||||
| 5 | VAL | ARG | ASP | LEU | LEU | ASP | ILE | LEU | ARG | GLU | ||||
| 6 | ARG | GLY | LYS | LEU | SER | VAL | GLY | ASP | LEU | ALA | ||||
| 7 | GLU | LEU | LEU | TYR | ARG | VAL | ARG | ARG | PHE | ASP | ||||
| 8 | LEU | LEU | LYS | ARG | ILE | LEU | LYS | MET | ASP | ARG | ||||
| 9 | LYS | ALA | VAL | GLU | THR | HIS | LEU | LEU | ARG | ASN | ||||
| 10 | PRO | HIS | LEU | VAL | SER | ASP | TYR | ARG | VAL | LEU | ||||
| 11 | MET | ALA | GLU | ILE | GLY | GLU | ASP | LEU | ASP | LYS | ||||
| 12 | SER | ASP | VAL | SER | SER | LEU | ILE | GLY | LEU | MET | ||||
| 13 | LYS | ASP | TYR | MET | GLY | ARG | GLY | LYS | ILE | SER | ||||
| 14 | LYS | GLU | LYS | SER | PHE | LEU | ASP | LEU | VAL | VAL | ||||
| 15 | GLU | LEU | GLU | LYS | LEU | ASN | LEU | VAL | ALA | PRO | ||||
| 16 | ASP | GLN | LEU | ASP | LEU | LEU | GLU | LYS | CYS | LEU | ||||
| 17 | LYS | ASN | ILE | HIS | ARG | ILE | ASP | LEU | LYS | THR | ||||
| 18 | LYS | ILE | GLN | LYS | TYR | LYS | GLN | LEU | GLU | HIS | ||||
| 19 | HIS | HIS | HIS | HIS | HIS |
Entity 2, C8-1a 8 residues - Formula weight is not available
| 1 | ASP | PHE | SER | ARG | ASN | LEU | TYR | ASP |
Samples:
sample_1: c-FLIPs, [U-99% 13C; U-99% 15N], 0.6 mM; C8-1a 4.2 mM
sample_2: C8-1a 10 mM; c-FLIPs, [U-100% 15N], 0.6 mM
sample_conditions_1: ionic strength: 110 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K
sample_conditions_2: ionic strength: 110 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 13C-15N Filtered NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H ROESY | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H 13C-15N Filtered NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts