BMRB Entry 50538
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50538
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Title: Backbone Assignments for the Human Interleukin-17F Homodimer PubMed: 33706174
Deposition date: 2020-10-28 Original release date: 2021-04-19
Authors: Veverka, Vaclav; Waters, Lorna; Muskett, Frederick; Taylor, Richard; Henry, Alistair; Carr, Mark
Citation: Waters, Lorna; Veverka, Vaclav; Strong, Sarah; Muskett, Frederick; Dedi, Neesha; Lawson, Alastair; Prosser, Christine; Taylor, Richard; Henry, Alistair; Carr, Mark. "Conformational dynamics in interleukin 17A and 17F functional complexes is a key determinant of receptor A affinity and specificity" Cytokine 142, 155476-155476 (2021).
Assembly members:
entity_1, polymer, 134 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32
Entity Sequences (FASTA):
entity_1: MRKIPKVGHTFFQKPESCPP
VPGGSMKLDIGIINENQRVS
MSRNIESRSTSPWNYTVTWD
PNRYPSEVVQAQCRNLGCIN
AQGKEDISMNSVPIQQETLV
VRRKHQGCSVSFQLEKVLVT
VGCTCVTPVIHHVQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 339 |
| 15N chemical shifts | 108 |
| 1H chemical shifts | 108 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IL-17F - protomer 1 | 1 |
| 2 | IL-17F - protomer 2 | 1 |
Entities:
Entity 1, IL-17F - protomer 1 134 residues - Formula weight is not available
Mature sequence of human IL-17F, with an additional N-terminal Met
| 1 | MET | ARG | LYS | ILE | PRO | LYS | VAL | GLY | HIS | THR | ||||
| 2 | PHE | PHE | GLN | LYS | PRO | GLU | SER | CYS | PRO | PRO | ||||
| 3 | VAL | PRO | GLY | GLY | SER | MET | LYS | LEU | ASP | ILE | ||||
| 4 | GLY | ILE | ILE | ASN | GLU | ASN | GLN | ARG | VAL | SER | ||||
| 5 | MET | SER | ARG | ASN | ILE | GLU | SER | ARG | SER | THR | ||||
| 6 | SER | PRO | TRP | ASN | TYR | THR | VAL | THR | TRP | ASP | ||||
| 7 | PRO | ASN | ARG | TYR | PRO | SER | GLU | VAL | VAL | GLN | ||||
| 8 | ALA | GLN | CYS | ARG | ASN | LEU | GLY | CYS | ILE | ASN | ||||
| 9 | ALA | GLN | GLY | LYS | GLU | ASP | ILE | SER | MET | ASN | ||||
| 10 | SER | VAL | PRO | ILE | GLN | GLN | GLU | THR | LEU | VAL | ||||
| 11 | VAL | ARG | ARG | LYS | HIS | GLN | GLY | CYS | SER | VAL | ||||
| 12 | SER | PHE | GLN | LEU | GLU | LYS | VAL | LEU | VAL | THR | ||||
| 13 | VAL | GLY | CYS | THR | CYS | VAL | THR | PRO | VAL | ILE | ||||
| 14 | HIS | HIS | VAL | GLN |
Samples:
sample_1: IL-17F dimer, [U-15N], 210 uM
sample_2: IL-17F dimer, [U-13C; U-15N; U-2H], 90 uM
sample_3: IL-17F dimer, [U-15N; U-2H], 200 uM
sample_conditions_1: ionic strength: 140 mM; pH: 6; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D TROSY- HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D TROSY-HN(CO)CACB | sample_2 | isotropic | sample_conditions_1 |
| 3D 15N/1H NOESY-HSQC | sample_3 | isotropic | sample_conditions_1 |
| 3D TROSY-HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - Data acquisition and processing
NMRPipe - Data processing
SPARKY - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance II 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts