BMRB Entry 50540
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50540
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Title: Backbone Assignments for the Human Interleukin-17AF Heterodimer PubMed: 33706174
Deposition date: 2020-10-29 Original release date: 2021-04-19
Authors: Waters, Lorna; Strong, Sarah; Muskett, Frederick; Prosser, Christine; Taylor, Richard; Henry, Alistair; Carr, Mark
Citation: Waters, Lorna; Veverka, Vaclav; Strong, Sarah; Muskett, Frederick; Dedi, Neesha; Lawson, Alastair; Prosser, Christine; Taylor, Richard; Henry, Alistair; Carr, Mark. "Conformational dynamics in interleukin 17A and 17F functional complexes is a key determinant of receptor A affinity and specificity" Cytokine 142, 155476-155476 (2021).
Assembly members:
entity_1, polymer, 153 residues, Formula weight is not available
entity_2, polymer, 133 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32
Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGENLYFQG
SGITIPRNPGCPNSEDKNFP
RTVMVNLNIHNRNTNTNPKR
SSDYYNRSTSPWNLHRNEDP
ERYPSVIWEAKCRHLGCINA
DGNVDYHMNSVPIQQEILVL
RREPPHCPNSFRLEKILVSV
GCTCVTPIVHHVA
entity_2: RKIPKVGHTFFQKPESCPPV
PGGSMKLDIGIINENQRVSM
SRNIESRSTSPWNYTVTWDP
NRYPSEVVQAQCRNLGCINA
QGKEDISMNSVPIQQETLVV
RRKHQGCSVSFQLEKVLVTV
GCTCVTPVIHHVQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 729 |
| 15N chemical shifts | 235 |
| 1H chemical shifts | 235 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IL-17A - protomer | 1 |
| 2 | IL-17F - protomer | 2 |
Entities:
Entity 1, IL-17A - protomer 153 residues - Formula weight is not available
Mature sequence of human IL-17A with N-terminal His-tag and linker
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | GLU | ASN | LEU | TYR | PHE | GLN | GLY | ||||
| 3 | SER | GLY | ILE | THR | ILE | PRO | ARG | ASN | PRO | GLY | ||||
| 4 | CYS | PRO | ASN | SER | GLU | ASP | LYS | ASN | PHE | PRO | ||||
| 5 | ARG | THR | VAL | MET | VAL | ASN | LEU | ASN | ILE | HIS | ||||
| 6 | ASN | ARG | ASN | THR | ASN | THR | ASN | PRO | LYS | ARG | ||||
| 7 | SER | SER | ASP | TYR | TYR | ASN | ARG | SER | THR | SER | ||||
| 8 | PRO | TRP | ASN | LEU | HIS | ARG | ASN | GLU | ASP | PRO | ||||
| 9 | GLU | ARG | TYR | PRO | SER | VAL | ILE | TRP | GLU | ALA | ||||
| 10 | LYS | CYS | ARG | HIS | LEU | GLY | CYS | ILE | ASN | ALA | ||||
| 11 | ASP | GLY | ASN | VAL | ASP | TYR | HIS | MET | ASN | SER | ||||
| 12 | VAL | PRO | ILE | GLN | GLN | GLU | ILE | LEU | VAL | LEU | ||||
| 13 | ARG | ARG | GLU | PRO | PRO | HIS | CYS | PRO | ASN | SER | ||||
| 14 | PHE | ARG | LEU | GLU | LYS | ILE | LEU | VAL | SER | VAL | ||||
| 15 | GLY | CYS | THR | CYS | VAL | THR | PRO | ILE | VAL | HIS | ||||
| 16 | HIS | VAL | ALA |
Entity 2, IL-17F - protomer 133 residues - Formula weight is not available
Mature sequence of human IL-17F
| 1 | ARG | LYS | ILE | PRO | LYS | VAL | GLY | HIS | THR | PHE | ||||
| 2 | PHE | GLN | LYS | PRO | GLU | SER | CYS | PRO | PRO | VAL | ||||
| 3 | PRO | GLY | GLY | SER | MET | LYS | LEU | ASP | ILE | GLY | ||||
| 4 | ILE | ILE | ASN | GLU | ASN | GLN | ARG | VAL | SER | MET | ||||
| 5 | SER | ARG | ASN | ILE | GLU | SER | ARG | SER | THR | SER | ||||
| 6 | PRO | TRP | ASN | TYR | THR | VAL | THR | TRP | ASP | PRO | ||||
| 7 | ASN | ARG | TYR | PRO | SER | GLU | VAL | VAL | GLN | ALA | ||||
| 8 | GLN | CYS | ARG | ASN | LEU | GLY | CYS | ILE | ASN | ALA | ||||
| 9 | GLN | GLY | LYS | GLU | ASP | ILE | SER | MET | ASN | SER | ||||
| 10 | VAL | PRO | ILE | GLN | GLN | GLU | THR | LEU | VAL | VAL | ||||
| 11 | ARG | ARG | LYS | HIS | GLN | GLY | CYS | SER | VAL | SER | ||||
| 12 | PHE | GLN | LEU | GLU | LYS | VAL | LEU | VAL | THR | VAL | ||||
| 13 | GLY | CYS | THR | CYS | VAL | THR | PRO | VAL | ILE | HIS | ||||
| 14 | HIS | VAL | GLN |
Samples:
sample_1: His-IL-17A, [U-13C; U-15N; U-2H], 260 uM; IL-17F 260 uM
sample_2: His-IL-17A 110 uM; IL-17F, [U-13C; U-15N; U-2H], 110 uM
sample_3: His-IL-17A 400 uM; IL-17F, [U-13C; U-15N; U-2H], 400 uM
sample_conditions_1: ionic strength: 140 mM; pH: 6; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D TROSY- HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D TROSY-HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 15N/1H NOESY-TROSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N TROSY | sample_2 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCACB | sample_3 | isotropic | sample_conditions_1 |
| 3D TROSY-HN(CO)CACB | sample_3 | isotropic | sample_conditions_1 |
| 3D 15N/1H NOESY-HSQC | sample_3 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - Data acquisition and processing
NMRPipe - Data processing and NUS reconstruction
NMRFAM-SPARKY - data analysis
NMR spectrometers:
- Bruker AVIII 600 MHz
- Bruker AVII 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts