BMRB Entry 50542

Name: Alpha-endosulfine (ENSA)
Published: 2021-08-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50542

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Alpha-endosulfine (ENSA) PubMed: 34346186

Deposition date: 2020-10-30 Original release date: 2021-08-10

Authors: Thapa, Chandan; Pentikainen, Ulla; Permi, Perttu

Citation: Thapa, Chandan; Roivas, Pekka; Haataja, Tatu; Permi, Perttu; Pentikainen, Ulla. "Interaction mechanism of endogenous PP2A inhibitor protein ENSA with PP2A" FEBS J. 289, 519-534 (2022).

Assembly members:
entity_1, polymer, 121 residues, 13000 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGTvL1-SGC

Entity Sequences (FASTA):
entity_1: MSQKQEEENPAEETGEEKQD TQEKEGILPERAEEAKLKAK YPSLGQKPGGSDFLMKRLQK GQKYFDSGDYNMAKAKMKNK QLPSAGPDKNLVTGDHIPTP QDLPQRKSSLVTSKLAGGQV E

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	349
15N chemical shifts	119
1H chemical shifts	239

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ENSA	1

Entities:

Entity 1, ENSA 121 residues - 13000 Da.

1

MET

SER

GLN

LYS

GLN

GLU

ASN

PRO

2

ALA

GLU

THR

GLY

GLU

LYS

GLN

ASP

3

THR

GLN

GLU

LYS

GLU

GLY

ILE

LEU

PRO

GLU

4

ARG

ALA

GLU

ALA

LYS

LEU

LYS

ALA

LYS

5

TYR

PRO

SER

LEU

GLY

GLN

LYS

PRO

GLY

6

SER

ASP

PHE

LEU

MET

LYS

ARG

LEU

GLN

LYS

7

GLY

GLN

LYS

TYR

PHE

ASP

SER

GLY

ASP

TYR

8

ASN

MET

ALA

LYS

ALA

LYS

MET

LYS

ASN

LYS

9

GLN

LEU

PRO

SER

ALA

GLY

PRO

ASP

LYS

ASN

10

LEU

VAL

THR

GLY

ASP

HIS

ILE

PRO

THR

PRO

11

GLN

ASP

LEU

PRO

GLN

ARG

LYS

SER

LEU

12

VAL

THR

SER

LYS

LEU

ALA

GLY

GLN

VAL

13

GLU

Samples:

sample_1: Alpha-endosulfine (ENSA), [U-98% 13C; U-98% 15N], 0.65 mM; Ammonium Chloride, [U-99% 15N], 1 g/L; D-glucose, [U-100% 13C], 2 g/L; ammonium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; D2O, [U-99% 2H], 5%

sample_2: Alpha-endosulfine (ENSA), [U-99% 15N], 0.65 mM; Ammonium Chloride, [U-99% 15N], 1 g/L; D-glucose, [U-100% 13C], 2 g/L; ammonium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; D2O, [U-99% 2H], 5%

sample_conditions_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D i(HCA)CO(CA)NH	sample_1	isotropic	sample_conditions_1
3D iHA(CA)NCO	sample_1	isotropic	sample_conditions_1
3D HA(CA)CON	sample_1	isotropic	sample_conditions_1
3D HA(CA)CON(CA)HA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0.8 - collection, processing

NMRFAM-SPARKY v1.4 - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts