BMRB Entry 50545
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50545
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Title: 1H, 13C and 15N NMR chemical shift assignment of A. thaliana RCD1 RST 487-589 PubMed: 34687712
Deposition date: 2020-11-04 Original release date: 2021-12-15
Authors: Staby, Lasse; Kragelund, Birthe
Citation: Staby, Lasse; Due, Amanda; Kunze, Micha; Jorgensen, Maria; Skriver, Karen; Kragelund, Birthe. "Flanking Disorder of the Folded aa-Hub Domain from Radical Induced Cell Death1 Affects Transcription Factor Binding by Ensemble Redistribution" J. Mol. Biol. 433, 167320-167320 (2021).
Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET-11a
Entity Sequences (FASTA):
entity_1: MARGSGSANSVGSSTTRPKS
PWMPFPTLFAAISHKVAEND
MLLINADYQQLRDKKMTRAE
FVRKLRVIVGDDLLRSTITT
LQNQPKSKEIPGSIRDHEEG
AGGL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 279 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 92 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RCD1-RST | 1 |
Entities:
Entity 1, RCD1-RST 104 residues - Formula weight is not available
| 1 | MET | ALA | ARG | GLY | SER | GLY | SER | ALA | ASN | SER | ||||
| 2 | VAL | GLY | SER | SER | THR | THR | ARG | PRO | LYS | SER | ||||
| 3 | PRO | TRP | MET | PRO | PHE | PRO | THR | LEU | PHE | ALA | ||||
| 4 | ALA | ILE | SER | HIS | LYS | VAL | ALA | GLU | ASN | ASP | ||||
| 5 | MET | LEU | LEU | ILE | ASN | ALA | ASP | TYR | GLN | GLN | ||||
| 6 | LEU | ARG | ASP | LYS | LYS | MET | THR | ARG | ALA | GLU | ||||
| 7 | PHE | VAL | ARG | LYS | LEU | ARG | VAL | ILE | VAL | GLY | ||||
| 8 | ASP | ASP | LEU | LEU | ARG | SER | THR | ILE | THR | THR | ||||
| 9 | LEU | GLN | ASN | GLN | PRO | LYS | SER | LYS | GLU | ILE | ||||
| 10 | PRO | GLY | SER | ILE | ARG | ASP | HIS | GLU | GLU | GLY | ||||
| 11 | ALA | GLY | GLY | LEU |
Samples:
sample_1: RCD1-RST, [U-100% 13C; U-100% 15N], 500 uM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 0.2%; DSS 0.7 mM
sample_conditions_1: ionic strength: 143 mM; pH: 7.0; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)N(CA)NNH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
qMDD - processing
NMRPipe - processing
TOPSPIN - collection
NMR spectrometers:
- Oxford INOVA 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts