BMRB Entry 50553
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50553
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Title: 15N relaxation measurements of an FG Nucleoporin in crowded conditions
Deposition date: 2020-11-09 Original release date: 2021-05-03
Authors: Hough, Loren; Maguire, Laura; Wall, Kathryn
Citation: Maguire, Laura; Wall, Kathryn; Hough, Loren. "Aggregation of an FG nucleoporin under crowded conditions" .
Assembly members:
entity_1, polymer, 133 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a
Entity Sequences (FASTA):
entity_1: MDNKTTNTTPSFSFGAKSDE
NKAGATSKPAFSFGAKPEEK
KDDNSSKPAFSFGAKSNEDK
QDGTAKPAFSFGAKPAEKNN
NETSKPAFSFGAKSDEKKDG
DASKPAFSFGAKPDENKASA
TSKPALEHHHHHH
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| T1 relaxation values | 203 |
| T2 relaxation values | 215 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 133 residues - Formula weight is not available
| 1 | MET | ASP | ASN | LYS | THR | THR | ASN | THR | THR | PRO | ||||
| 2 | SER | PHE | SER | PHE | GLY | ALA | LYS | SER | ASP | GLU | ||||
| 3 | ASN | LYS | ALA | GLY | ALA | THR | SER | LYS | PRO | ALA | ||||
| 4 | PHE | SER | PHE | GLY | ALA | LYS | PRO | GLU | GLU | LYS | ||||
| 5 | LYS | ASP | ASP | ASN | SER | SER | LYS | PRO | ALA | PHE | ||||
| 6 | SER | PHE | GLY | ALA | LYS | SER | ASN | GLU | ASP | LYS | ||||
| 7 | GLN | ASP | GLY | THR | ALA | LYS | PRO | ALA | PHE | SER | ||||
| 8 | PHE | GLY | ALA | LYS | PRO | ALA | GLU | LYS | ASN | ASN | ||||
| 9 | ASN | GLU | THR | SER | LYS | PRO | ALA | PHE | SER | PHE | ||||
| 10 | GLY | ALA | LYS | SER | ASP | GLU | LYS | LYS | ASP | GLY | ||||
| 11 | ASP | ALA | SER | LYS | PRO | ALA | PHE | SER | PHE | GLY | ||||
| 12 | ALA | LYS | PRO | ASP | GLU | ASN | LYS | ALA | SER | ALA | ||||
| 13 | THR | SER | LYS | PRO | ALA | LEU | GLU | HIS | HIS | HIS | ||||
| 14 | HIS | HIS | HIS |
Samples:
sample_1: FSFG-K-6His, [U-100% 15N], 250 uM; H2O 90%; D2O 10%; HEPES 20 mM; KCL 150 mM; MgCl2 2 mM; PEG 13 % w/v
sample_2: FSFG-K-6His, [U-100% 13C; U-100% 15N], 250 uM; H2O 90%; D2O 10%; HEPES 20 mM; KCL 150 mM; MgCl2 2 mM; PVP 13 % w/v
sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| T2/R2 relaxation | sample_1 | isotropic | sample_conditions_1 |
| T1/R1 relaxation | sample_2 | isotropic | sample_conditions_1 |
Software:
CcpNMR - NMR relaxation time constant
NMR spectrometers:
- Varian INOVA 600 MHz