BMRB Entry 50564
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50564
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Title: Mutation G269S enhances resistance of Mycobacterium tuberculosis b-lactamase against sulbactam PubMed: 34250791
Deposition date: 2020-11-11 Original release date: 2021-07-01
Authors: van Alen, Ilona; Ubbink, Marcellus
Citation: van Alen, Ilona; Chikunova, Aleksandra; Safeer, Adil; Ud Din Ahmad, Misbha; Perrakis, Anastassis; Ubbink, Marcellus. "The G132S mutation enhances the resistance of Mycobacterium tuberculosis beta-lactamase against sulbactam" Biochemistry 60, 2236-2245 (2021).
Assembly members:
entity_1, polymer, 266 residues, 28331 Da.
Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: GDLADRFAELERRYDARLGV
YVPATGTTAAIEYRADERFA
FCSTFKAPLVAAVLHQNPLT
HLDKLITYTSDDIRSISPVA
QQHVQTGMTIGQLCDAAIRY
SDGTAANLLLADLGGPGGGT
AAFTGYLRSLGDTVSRLDAE
EPELNRDPPGDERDTTTPHA
IALVLQQLVLGNALPPDKRA
LLTDWMARNTTGAKRIRAGF
PADWKVIDKTGTGDYGRAND
IAVVWSPTGVPYVVAVMSDR
ASGGYDAEPREALLAEAATC
VAGVLA
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 245 |
| 1H chemical shifts | 245 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BlaC G269S | 1 |
Entities:
Entity 1, BlaC G269S 266 residues - 28331 Da.
Ambler standardised numbering for class A beta-lactamases
| 1 | GLY | ASP | LEU | ALA | ASP | ARG | PHE | ALA | GLU | LEU | ||||
| 2 | GLU | ARG | ARG | TYR | ASP | ALA | ARG | LEU | GLY | VAL | ||||
| 3 | TYR | VAL | PRO | ALA | THR | GLY | THR | THR | ALA | ALA | ||||
| 4 | ILE | GLU | TYR | ARG | ALA | ASP | GLU | ARG | PHE | ALA | ||||
| 5 | PHE | CYS | SER | THR | PHE | LYS | ALA | PRO | LEU | VAL | ||||
| 6 | ALA | ALA | VAL | LEU | HIS | GLN | ASN | PRO | LEU | THR | ||||
| 7 | HIS | LEU | ASP | LYS | LEU | ILE | THR | TYR | THR | SER | ||||
| 8 | ASP | ASP | ILE | ARG | SER | ILE | SER | PRO | VAL | ALA | ||||
| 9 | GLN | GLN | HIS | VAL | GLN | THR | GLY | MET | THR | ILE | ||||
| 10 | GLY | GLN | LEU | CYS | ASP | ALA | ALA | ILE | ARG | TYR | ||||
| 11 | SER | ASP | GLY | THR | ALA | ALA | ASN | LEU | LEU | LEU | ||||
| 12 | ALA | ASP | LEU | GLY | GLY | PRO | GLY | GLY | GLY | THR | ||||
| 13 | ALA | ALA | PHE | THR | GLY | TYR | LEU | ARG | SER | LEU | ||||
| 14 | GLY | ASP | THR | VAL | SER | ARG | LEU | ASP | ALA | GLU | ||||
| 15 | GLU | PRO | GLU | LEU | ASN | ARG | ASP | PRO | PRO | GLY | ||||
| 16 | ASP | GLU | ARG | ASP | THR | THR | THR | PRO | HIS | ALA | ||||
| 17 | ILE | ALA | LEU | VAL | LEU | GLN | GLN | LEU | VAL | LEU | ||||
| 18 | GLY | ASN | ALA | LEU | PRO | PRO | ASP | LYS | ARG | ALA | ||||
| 19 | LEU | LEU | THR | ASP | TRP | MET | ALA | ARG | ASN | THR | ||||
| 20 | THR | GLY | ALA | LYS | ARG | ILE | ARG | ALA | GLY | PHE | ||||
| 21 | PRO | ALA | ASP | TRP | LYS | VAL | ILE | ASP | LYS | THR | ||||
| 22 | GLY | THR | GLY | ASP | TYR | GLY | ARG | ALA | ASN | ASP | ||||
| 23 | ILE | ALA | VAL | VAL | TRP | SER | PRO | THR | GLY | VAL | ||||
| 24 | PRO | TYR | VAL | VAL | ALA | VAL | MET | SER | ASP | ARG | ||||
| 25 | ALA | SER | GLY | GLY | TYR | ASP | ALA | GLU | PRO | ARG | ||||
| 26 | GLU | ALA | LEU | LEU | ALA | GLU | ALA | ALA | THR | CYS | ||||
| 27 | VAL | ALA | GLY | VAL | LEU | ALA |
Samples:
sample_1: BlaC G269S, [U-100% 15N], 0.15 mM; NaPi, naturan abundance, 100 mM
sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.0.6 - collection, processing
CcpNmr Analysis v2.4.0 - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts