BMRB Entry 50595

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50595

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone and aliphatic side chain of Gamma mini-lid R133A
Deposition date:
2020-11-26
Original release date:
2021-06-30
Authors:
Mahdi, Sam; Bezsonova, Irina; Beuning, Penny; Korzhnev, Dmitry
Citation:

Citation: Mahdi, Sam; Bezsonova, Irina; Beuning, Penny; Korzhnev, Dmitry. "NMR resonance assignments for the nucleotide binding domains of the E. coli clamp loader complex Gamma subunit"  Biomol. NMR Assign. 15, 281-285 (2021).
PubMed: 33761093

Assembly members:

Assembly members:
entity_1, polymer, 243 residues, 26764.61 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMSYQVLARKWRPQTFADVV GQEHVLTALANGLSLGRIHH AYLFSGTRGVGKTSIARLLA KGLNCETGITATPCGVCDNC REIEQGRFVDLIEIDAASRT KVEDTRDLLDNVQYAPARGR FKVYLIDEVHMLSAHSFNAL LKTLEEPPEHVKFLLATTDP QKLPVTILSRCLQFHLKALD VEQIRHQLEHILNEEHIAHE PRALQLLARAAEGSLRDALS LTDQAIASGDGQVSTQAVSA MLG

Data typeCount
13C chemical shifts870
15N chemical shifts214
1H chemical shifts1156

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Gamma Mini-Lid R133A1

Entities:

Entity 1, Gamma Mini-Lid R133A 243 residues - 26764.61 Da.

Sequence is mutated form of Wild Type (R133A)

1   SERMETSERTYRGLNVALLEUALAARGLYS
2   TRPARGPROGLNTHRPHEALAASPVALVAL
3   GLYGLNGLUHISVALLEUTHRALALEUALA
4   ASNGLYLEUSERLEUGLYARGILEHISHIS
5   ALATYRLEUPHESERGLYTHRARGGLYVAL
6   GLYLYSTHRSERILEALAARGLEULEUALA
7   LYSGLYLEUASNCYSGLUTHRGLYILETHR
8   ALATHRPROCYSGLYVALCYSASPASNCYS
9   ARGGLUILEGLUGLNGLYARGPHEVALASP
10   LEUILEGLUILEASPALAALASERARGTHR
11   LYSVALGLUASPTHRARGASPLEULEUASP
12   ASNVALGLNTYRALAPROALAARGGLYARG
13   PHELYSVALTYRLEUILEASPGLUVALHIS
14   METLEUSERALAHISSERPHEASNALALEU
15   LEULYSTHRLEUGLUGLUPROPROGLUHIS
16   VALLYSPHELEULEUALATHRTHRASPPRO
17   GLNLYSLEUPROVALTHRILELEUSERARG
18   CYSLEUGLNPHEHISLEULYSALALEUASP
19   VALGLUGLNILEARGHISGLNLEUGLUHIS
20   ILELEUASNGLUGLUHISILEALAHISGLU
21   PROARGALALEUGLNLEULEUALAARGALA
22   ALAGLUGLYSERLEUARGASPALALEUSER
23   LEUTHRASPGLNALAILEALASERGLYASP
24   GLYGLNVALSERTHRGLNALAVALSERALA
25   METLEUGLY

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks