BMRB Entry 50626

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50626

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Title:
Backbone chemical shift assignments for the D3 translational isoform of the human glucocorticoid receptor
Deposition date:
2020-12-09
Original release date:
2021-05-07
Authors:
Grasso, Emily; Majumdar, Ananya; Wrabl, James; Frueh, Dominique; Hilser, Vincent
Citation:

Citation: Grasso, Emily; Majumdar, Ananya; Wrabl, James; Frueh, Dominique; Hilser, Vincent. "Conserved allosteric ensembles in a disordered protein using TROSY/Anti-TROSY R 2-filtered spectroscopy"  Biophys. J. 120, 2498-2510 (2021).
PubMed: 33901472

Assembly members:

Assembly members:
entity_1, polymer, 193 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pj411

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMNTASLSQQQDQKPIFN VIPPIPVGSENWNRCQGSGD DNLTSLGTLNFPGRTVFSNG YSSPSMRPDVSSPPSSSSTA TTGPPPKLCLVCSDEASGCH YGVLTCGSCKVFFKRAVEGQ HNYLCAGRNDCIIDKIRRKN CPACRYRKCLQAGMNLEARK TKKKIKGIQQATTGVSQETS ENPGNKTIVPATL

Data sets:
Data typeCount
13C chemical shifts495
15N chemical shifts166
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2zinc 12
3zinc 22

Entities:

Entity 1, protein 193 residues - Formula weight is not available

The initial 3 amino acids remain after cleavage of N-terminal his tag; residue 336 is the initial M in the sequence.

1   GLYSERHISMETASNTHRALASERLEUSER
2   GLNGLNGLNASPGLNLYSPROILEPHEASN
3   VALILEPROPROILEPROVALGLYSERGLU
4   ASNTRPASNARGCYSGLNGLYSERGLYASP
5   ASPASNLEUTHRSERLEUGLYTHRLEUASN
6   PHEPROGLYARGTHRVALPHESERASNGLY
7   TYRSERSERPROSERMETARGPROASPVAL
8   SERSERPROPROSERSERSERSERTHRALA
9   THRTHRGLYPROPROPROLYSLEUCYSLEU
10   VALCYSSERASPGLUALASERGLYCYSHIS
11   TYRGLYVALLEUTHRCYSGLYSERCYSLYS
12   VALPHEPHELYSARGALAVALGLUGLYGLN
13   HISASNTYRLEUCYSALAGLYARGASNASP
14   CYSILEILEASPLYSILEARGARGLYSASN
15   CYSPROALACYSARGTYRARGLYSCYSLEU
16   GLNALAGLYMETASNLEUGLUALAARGLYS
17   THRLYSLYSLYSILELYSGLYILEGLNGLN
18   ALATHRTHRGLYVALSERGLNGLUTHRSER
19   GLUASNPROGLYASNLYSTHRILEVALPRO
20   ALATHRLEU

Entity 2, zinc 1 - Zn - 65.409 Da.

1   ZN

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks