BMRB Entry 50631

Name: Hinge region of the Trypanosoma brucei PKA regulatory subunit
Published: 2021-01-25

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50631

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Hinge region of the Trypanosoma brucei PKA regulatory subunit PubMed: 33359563

Deposition date: 2020-12-09 Original release date: 2021-01-25

Authors: Araujo, Nelson; Bruix, Marta; Laurents, Douglas

Citation: Araujo, Nelson; Bruix, Marta; Laurents, Douglas. "Disorder and partial folding in the regulatory subunit hinge region of Trypanosoma brucei protein kinase A: The C-linker portion inhibits the parasite's protein kinase A" Arch. Biochem. Biophys. 698, 108731-108731 (2021).

Assembly members:
entity_1, polymer, 26 residues, Formula weight is not available

Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei

Experimental source: Production method: Synthetic peptides

Entity Sequences (FASTA):
entity_1: GGRKRRTTVRGEGIDPEKAK SYVAPY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_3

Data type	Count
13C chemical shifts	48
1H chemical shifts	170

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Hinge region	1

Entities:

Entity 1, Hinge region 26 residues - Formula weight is not available

1

GLY

ARG

LYS

ARG

THR

VAL

ARG

2

GLY

GLU

GLY

ILE

ASP

PRO

GLU

LYS

ALA

LYS

3

SER

TYR

VAL

ALA

PRO

TYR

Samples:

sample_1: Hinge region of the PKA regulatory subunit 1.9 mM; DSS 0.04 mM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1 - Data acquisition

SPARKY v3.114 - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz