BMRB Entry 50637
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50637
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Citation: Dagenais, Pierre; Desjardins, Genevieve; Legault, Pascale. "An integrative NMR-SAXS approach for structural determination of large RNAs defines the substrate-free state of a trans-cleaving Neurospora Varkud Satellite ribozyme" Nucleic Acids Res. 49, 11959-11973 (2021).
PubMed: 34718697
Assembly members:
entity_1, polymer, 101 residues, 32652.55626 Da.
Natural source: Common Name: Neurospora crassa Taxonomy ID: 5141 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Neurospora crassa
Experimental source: Production method: recombinant technology Host organism: Synthetic Vector: pTZ19R-derived plasmid
Entity Sequences (FASTA):
entity_1: GCAGGGAACUCACCUCCCCU
UGGGUCGUAGCAGUUGACUA
CUGUUAUGUGAUUGGUAGAG
GCUAAGUGACGCCGUAAGGC
GCAGCACAGCACAAGCCCGC
G
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 15N chemical shifts | 78 |
| 1H chemical shifts | 78 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Minimal trans VS ribozyme | 1 |
Entities:
Entity 1, Minimal trans VS ribozyme 101 residues - 32652.55626 Da.
| 1 | G | C | A | G | G | G | A | A | C | U | ||||
| 2 | C | A | C | C | U | C | C | C | C | U | ||||
| 3 | U | G | G | G | U | C | G | U | A | G | ||||
| 4 | C | A | G | U | U | G | A | C | U | A | ||||
| 5 | C | U | G | U | U | A | U | G | U | G | ||||
| 6 | A | U | U | G | G | U | A | G | A | G | ||||
| 7 | G | C | U | A | A | G | U | G | A | C | ||||
| 8 | G | C | C | G | U | A | A | G | G | C | ||||
| 9 | G | C | A | G | C | A | C | A | G | C | ||||
| 10 | A | C | A | A | G | C | C | C | G | C | ||||
| 11 | G |