BMRB Entry 50637
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50637
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Title: Minimal trans VS ribozyme in 5 mM MgCl2 PubMed: 34718697
Deposition date: 2020-12-11 Original release date: 2021-10-06
Authors: Dagenais, Pierre; Desjardins, Genevieve; Legault, Pascale
Citation: Dagenais, Pierre; Desjardins, Genevieve; Legault, Pascale. "An integrative NMR-SAXS approach for structural determination of large RNAs defines the substrate-free state of a trans-cleaving Neurospora Varkud Satellite ribozyme" Nucleic Acids Res. 49, 11959-11973 (2021).
Assembly members:
entity_1, polymer, 101 residues, 32652.55626 Da.
Natural source: Common Name: Neurospora crassa Taxonomy ID: 5141 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Neurospora crassa
Experimental source: Production method: recombinant technology Host organism: Synthetic Vector: pTZ19R-derived plasmid
Entity Sequences (FASTA):
entity_1: GCAGGGAACUCACCUCCCCU
UGGGUCGUAGCAGUUGACUA
CUGUUAUGUGAUUGGUAGAG
GCUAAGUGACGCCGUAAGGC
GCAGCACAGCACAAGCCCGC
G
- assigned_chemical_shifts
- spectral_peak_list
Data type | Count |
15N chemical shifts | 78 |
1H chemical shifts | 78 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Minimal trans VS ribozyme | 1 |
Entities:
Entity 1, Minimal trans VS ribozyme 101 residues - 32652.55626 Da.
1 | G | C | A | G | G | G | A | A | C | U | ||||
2 | C | A | C | C | U | C | C | C | C | U | ||||
3 | U | G | G | G | U | C | G | U | A | G | ||||
4 | C | A | G | U | U | G | A | C | U | A | ||||
5 | C | U | G | U | U | A | U | G | U | G | ||||
6 | A | U | U | G | G | U | A | G | A | G | ||||
7 | G | C | U | A | A | G | U | G | A | C | ||||
8 | G | C | C | G | U | A | A | G | G | C | ||||
9 | G | C | A | G | C | A | C | A | G | C | ||||
10 | A | C | A | A | G | C | C | C | G | C | ||||
11 | G |
Samples:
sample_1: TR4P, [U-15N], 1.7 mM; Na cacodylate 10 mM; KCl 50 mM; MgCl2 5 mM; NaN3 0.05 mM
sample_conditions_1: pH: 6.500; pressure: 1.000 atm; temperature: 288.000 K
sample_conditions_2: pH: 6.500; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC/HMQC | sample_1 | isotropic | sample_conditions_2 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_2 |
Software:
CcpNmr Analysis v2.5 - Chemical shift assignments, data analysis
NMRPipe v9.7 - Data processing, data analysis
NMR spectrometers:
- Bruker Avance 700 MHz