BMRB Entry 50686

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50686

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Title:
Backbone chemical shift assignment, R1 and R2 relaxation rates of R272E/K273E mutant of deubiquitinase A
Deposition date:
2020-12-24
Original release date:
2021-02-13
Authors:
Kabra, Ashish; Li, Ying
Citation:

Citation: Kabra, Ashish; Li, Ying. "Conformational Dynamics of Deubiquitinase A and Functional Implications"  Biochemistry 69, 201-209 (2021).
PubMed: 33417762

Assembly members:

Assembly members:
entity_1, polymer, 168 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28-MHL

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAGYNSEDEYEAAAARIEAM DPATVEQQEHWFEKALRDKK GFIIKQMKEDGACLFRAVAD QVYGDQDMHEVVRKHCMDYL MKNADYFSNYVTEDFTTYIN EERKNNCHGNHIEMQAMAEM YNRPVEVYQYSTGTSAVEPI NTFHGIHQNEDEPIRVSYHR NIHYNSVVNPNKA

Data sets:
Data typeCount
13C chemical shifts455
15N chemical shifts144
1H chemical shifts144
T1 relaxation values141
T2 relaxation values141

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1DUBA R272E/K273E mutant1

Entities:

Entity 1, DUBA R272E/K273E mutant 168 residues - Formula weight is not available

1   GLYALAGLYTYRASNSERGLUASPGLUTYR
2   GLUALAALAALAALAARGILEGLUALAMET
3   ASPPROALATHRVALGLUGLNGLNGLUHIS
4   TRPPHEGLULYSALALEUARGASPLYSLYS
5   GLYPHEILEILELYSGLNMETLYSGLUASP
6   GLYALACYSLEUPHEARGALAVALALAASP
7   GLNVALTYRGLYASPGLNASPMETHISGLU
8   VALVALARGLYSHISCYSMETASPTYRLEU
9   METLYSASNALAASPTYRPHESERASNTYR
10   VALTHRGLUASPPHETHRTHRTYRILEASN
11   GLUGLUARGLYSASNASNCYSHISGLYASN
12   HISILEGLUMETGLNALAMETALAGLUMET
13   TYRASNARGPROVALGLUVALTYRGLNTYR
14   SERTHRGLYTHRSERALAVALGLUPROILE
15   ASNTHRPHEHISGLYILEHISGLNASNGLU
16   ASPGLUPROILEARGVALSERTYRHISARG
17   ASNILEHISTYRASNSERVALVALASNPRO
18   ASNLYSALA

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks