BMRB Entry 50689

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50689

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Title:
Chemical shift assignments of the Campylobacter jejuni helical cell shape determining protein Pgp2 in its apo form
Deposition date:
2021-01-04
Original release date:
2021-07-27
Authors:
Lin, Chang Sheng-Huei; McIntosh, Lawrence; Murphy, Michael
Citation:

Citation: Lin, Chang Sheng-Huei; Chan, Anson; Vermeulen, Jenny; Brockerman, Jacob; Soni, Arvind; Tanner, Martin; Gaynor, Erin; Mclntosh, Lawrence; Simorre, Jean-Pierre; Murphy, Michael. "Peptidoglycan binding by a pocket on the accessory NTF2-domain of Pgp2 directs helical cell shape of Campylobacter jejuni"  J. Biol. Chem. 296, 100528-100528 (2021).
PubMed: 33711341

Assembly members:

Assembly members:
entity_1, polymer, 287 residues, 33604 Da.

Natural source:

Natural source:   Common Name: Campylobacter jejuni   Taxonomy ID: 197   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Campylobacter jejuni

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMQKDFWLSEIGDKNISL GYYDDNVAIVLTNKTDKILR VYSYEDGKIRKDFEQKEIIT GLMGDKKIEGDLKTPVGFYE LGRKFNPGDPYYGPFAFATT YPNLLDKVQGKTGGGIWIHG YPLDGSRLDEFKTRGCIALF NNNLEKFAQVVQDKKVFVMT EEKEKIRAKKDQIASLLADL FTWKLAWTNSDTNTYLSFYD EQEFKRFDKMKFEQFASMKK SIFSRKEDKKIKFSDINISP YPNLENETMYRISFYEDYYT KNYQFRGDKILYVKIDSKGK MKILAEQ

Data sets:
Data typeCount
13C chemical shifts755
15N chemical shifts242
1H chemical shifts242

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Apo-Pgp2 monomer1

Entities:

Entity 1, Apo-Pgp2 monomer 287 residues - 33604 Da.

Residues 1-4 represent a non-native affinity tag

1   GLYSERHISMETGLNLYSASPPHETRPLEU
2   SERGLUILEGLYASPLYSASNILESERLEU
3   GLYTYRTYRASPASPASNVALALAILEVAL
4   LEUTHRASNLYSTHRASPLYSILELEUARG
5   VALTYRSERTYRGLUASPGLYLYSILEARG
6   LYSASPPHEGLUGLNLYSGLUILEILETHR
7   GLYLEUMETGLYASPLYSLYSILEGLUGLY
8   ASPLEULYSTHRPROVALGLYPHETYRGLU
9   LEUGLYARGLYSPHEASNPROGLYASPPRO
10   TYRTYRGLYPROPHEALAPHEALATHRTHR
11   TYRPROASNLEULEUASPLYSVALGLNGLY
12   LYSTHRGLYGLYGLYILETRPILEHISGLY
13   TYRPROLEUASPGLYSERARGLEUASPGLU
14   PHELYSTHRARGGLYCYSILEALALEUPHE
15   ASNASNASNLEUGLULYSPHEALAGLNVAL
16   VALGLNASPLYSLYSVALPHEVALMETTHR
17   GLUGLULYSGLULYSILEARGALALYSLYS
18   ASPGLNILEALASERLEULEUALAASPLEU
19   PHETHRTRPLYSLEUALATRPTHRASNSER
20   ASPTHRASNTHRTYRLEUSERPHETYRASP
21   GLUGLNGLUPHELYSARGPHEASPLYSMET
22   LYSPHEGLUGLNPHEALASERMETLYSLYS
23   SERILEPHESERARGLYSGLUASPLYSLYS
24   ILELYSPHESERASPILEASNILESERPRO
25   TYRPROASNLEUGLUASNGLUTHRMETTYR
26   ARGILESERPHETYRGLUASPTYRTYRTHR
27   LYSASNTYRGLNPHEARGGLYASPLYSILE
28   LEUTYRVALLYSILEASPSERLYSGLYLYS
29   METLYSILELEUALAGLUGLN

Related Database Links:

NCBI EAQ72310
PDB 6XJ6

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