BMRB Entry 50701
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50701
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Title: htau40 assignment PubMed: 20687558
Deposition date: 2021-01-07 Original release date: 2021-02-11
Authors: Zweckstetter, Anna Siegert
Citation: Narayanan, Rhagavendran; Durr, Ulrich; Bibow, Stefan; Biernat, Jacek; Mandelkow, Eckhard; Zweckstetter, Markus. "Automatic assignment of the intrinsically disordered protein Tau with 441-residues." J. Am. Chem. Soc. 132, 11906-11907 (2010).
Assembly members:
entity_1, polymer, 441 residues, 45849.91 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pNG2
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 362 |
| 1H chemical shifts | 362 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | htau40 | 1 |
Entities:
Entity 1, htau40 441 residues - 45849.91 Da.
| 1 | MET | ALA | GLU | PRO | ARG | GLN | GLU | PHE | GLU | VAL | ||||
| 2 | MET | GLU | ASP | HIS | ALA | GLY | THR | TYR | GLY | LEU | ||||
| 3 | GLY | ASP | ARG | LYS | ASP | GLN | GLY | GLY | TYR | THR | ||||
| 4 | MET | HIS | GLN | ASP | GLN | GLU | GLY | ASP | THR | ASP | ||||
| 5 | ALA | GLY | LEU | LYS | GLU | SER | PRO | LEU | GLN | THR | ||||
| 6 | PRO | THR | GLU | ASP | GLY | SER | GLU | GLU | PRO | GLY | ||||
| 7 | SER | GLU | THR | SER | ASP | ALA | LYS | SER | THR | PRO | ||||
| 8 | THR | ALA | GLU | ASP | VAL | THR | ALA | PRO | LEU | VAL | ||||
| 9 | ASP | GLU | GLY | ALA | PRO | GLY | LYS | GLN | ALA | ALA | ||||
| 10 | ALA | GLN | PRO | HIS | THR | GLU | ILE | PRO | GLU | GLY | ||||
| 11 | THR | THR | ALA | GLU | GLU | ALA | GLY | ILE | GLY | ASP | ||||
| 12 | THR | PRO | SER | LEU | GLU | ASP | GLU | ALA | ALA | GLY | ||||
| 13 | HIS | VAL | THR | GLN | ALA | ARG | MET | VAL | SER | LYS | ||||
| 14 | SER | LYS | ASP | GLY | THR | GLY | SER | ASP | ASP | LYS | ||||
| 15 | LYS | ALA | LYS | GLY | ALA | ASP | GLY | LYS | THR | LYS | ||||
| 16 | ILE | ALA | THR | PRO | ARG | GLY | ALA | ALA | PRO | PRO | ||||
| 17 | GLY | GLN | LYS | GLY | GLN | ALA | ASN | ALA | THR | ARG | ||||
| 18 | ILE | PRO | ALA | LYS | THR | PRO | PRO | ALA | PRO | LYS | ||||
| 19 | THR | PRO | PRO | SER | SER | GLY | GLU | PRO | PRO | LYS | ||||
| 20 | SER | GLY | ASP | ARG | SER | GLY | TYR | SER | SER | PRO | ||||
| 21 | GLY | SER | PRO | GLY | THR | PRO | GLY | SER | ARG | SER | ||||
| 22 | ARG | THR | PRO | SER | LEU | PRO | THR | PRO | PRO | THR | ||||
| 23 | ARG | GLU | PRO | LYS | LYS | VAL | ALA | VAL | VAL | ARG | ||||
| 24 | THR | PRO | PRO | LYS | SER | PRO | SER | SER | ALA | LYS | ||||
| 25 | SER | ARG | LEU | GLN | THR | ALA | PRO | VAL | PRO | MET | ||||
| 26 | PRO | ASP | LEU | LYS | ASN | VAL | LYS | SER | LYS | ILE | ||||
| 27 | GLY | SER | THR | GLU | ASN | LEU | LYS | HIS | GLN | PRO | ||||
| 28 | GLY | GLY | GLY | LYS | VAL | GLN | ILE | ILE | ASN | LYS | ||||
| 29 | LYS | LEU | ASP | LEU | SER | ASN | VAL | GLN | SER | LYS | ||||
| 30 | CYS | GLY | SER | LYS | ASP | ASN | ILE | LYS | HIS | VAL | ||||
| 31 | PRO | GLY | GLY | GLY | SER | VAL | GLN | ILE | VAL | TYR | ||||
| 32 | LYS | PRO | VAL | ASP | LEU | SER | LYS | VAL | THR | SER | ||||
| 33 | LYS | CYS | GLY | SER | LEU | GLY | ASN | ILE | HIS | HIS | ||||
| 34 | LYS | PRO | GLY | GLY | GLY | GLN | VAL | GLU | VAL | LYS | ||||
| 35 | SER | GLU | LYS | LEU | ASP | PHE | LYS | ASP | ARG | VAL | ||||
| 36 | GLN | SER | LYS | ILE | GLY | SER | LEU | ASP | ASN | ILE | ||||
| 37 | THR | HIS | VAL | PRO | GLY | GLY | GLY | ASN | LYS | LYS | ||||
| 38 | ILE | GLU | THR | HIS | LYS | LEU | THR | PHE | ARG | GLU | ||||
| 39 | ASN | ALA | LYS | ALA | LYS | THR | ASP | HIS | GLY | ALA | ||||
| 40 | GLU | ILE | VAL | TYR | LYS | SER | PRO | VAL | VAL | SER | ||||
| 41 | GLY | ASP | THR | SER | PRO | ARG | HIS | LEU | SER | ASN | ||||
| 42 | VAL | SER | SER | THR | GLY | SER | ILE | ASP | MET | VAL | ||||
| 43 | ASP | SER | PRO | GLN | LEU | ALA | THR | LEU | ALA | ASP | ||||
| 44 | GLU | VAL | SER | ALA | SER | LEU | ALA | LYS | GLN | GLY | ||||
| 45 | LEU |
Samples:
sample_1: Microtubule-associated protein tau, [U-100% 13C; U-100% 15N], 1.3 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| APSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v9.9 - chemical shift assignment, chemical shift calculation
MARS - chemical shift assignment
PROSA - data analysis
NMR spectrometers:
- Bruker AVANCE III 900 MHz
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts