BMRB Entry 50702
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50702
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Title: Degron-tagged Ig2D5 PubMed: 33929321
Deposition date: 2021-01-08 Original release date: 2021-05-18
Authors: Tzeng, Shiou-Ru
Citation: Tzeng, Shiou-Ru; Tseng, Yin-Chu; Lin, Chien-Chu; Hsu, Chia-Ying; Huang, Shing-Jong; Kuo, Yi-Ting; Chang, Chung-I. "Molecular insights into substrate recognition and discrimination by the N-terminal domain of Lon AAA+ protease" elife 10, e64056-e64056 (2021).
Assembly members:
entity_1, polymer, 130 residues, Formula weight is not available
Natural source: Common Name: Dictyostelium discoideum Taxonomy ID: 44689 Superkingdom: Eukaryota Kingdom: not available Genus/species: Dictyostelium discoideum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET_Ig2D5_S20
Entity Sequences (FASTA):
entity_1: GHMTVKPAPSAEHSYAEGEG
LVKVFDNAPAEFTIFAVDTK
GVARTDGGDPFEVAINGPDG
LVVDAKVTDNNDGTYGVVYD
APVEGNYNVNVTLRGNPIKN
MPIDVKCIEGASSHATRQLS
GLKIHSNLYH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 215 |
| 15N chemical shifts | 115 |
| 1H chemical shifts | 115 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Degron tag Sul20 | 1 |
Entities:
Entity 1, Degron tag Sul20 130 residues - Formula weight is not available
A degron-tagged Ig2D5 is a chimeric protein: Sul20 (the C-terminal 20 residues of SulA; namely Sul20) is appended to the C terminus of a protein substrate Ig2D5 (domains 5 of the gelation factor from Dictyostelium discoideum. The sequence of Ig2D5: GHMTVKPAPSAEHSYAEGEGLVKVFDNAPAEFTIFAVDTKGVARTDGGDPFEVAINGPDGLVVDAKVTDNNDGTYGVVYDAPVEGNYNVNVTLRGNPIKNMPIDVKCIEG The sequence of Sul20: ASSHATRQLSGLKIHSNLYH
| 1 | GLY | HIS | MET | THR | VAL | LYS | PRO | ALA | PRO | SER | |
| 2 | ALA | GLU | HIS | SER | TYR | ALA | GLU | GLY | GLU | GLY | |
| 3 | LEU | VAL | LYS | VAL | PHE | ASP | ASN | ALA | PRO | ALA | |
| 4 | GLU | PHE | THR | ILE | PHE | ALA | VAL | ASP | THR | LYS | |
| 5 | GLY | VAL | ALA | ARG | THR | ASP | GLY | GLY | ASP | PRO | |
| 6 | PHE | GLU | VAL | ALA | ILE | ASN | GLY | PRO | ASP | GLY | |
| 7 | LEU | VAL | VAL | ASP | ALA | LYS | VAL | THR | ASP | ASN | |
| 8 | ASN | ASP | GLY | THR | TYR | GLY | VAL | VAL | TYR | ASP | |
| 9 | ALA | PRO | VAL | GLU | GLY | ASN | TYR | ASN | VAL | ASN | |
| 10 | VAL | THR | LEU | ARG | GLY | ASN | PRO | ILE | LYS | ASN | |
| 11 | MET | PRO | ILE | ASP | VAL | LYS | CYS | ILE | GLU | GLY | |
| 12 | ALA | SER | SER | HIS | ALA | THR | ARG | GLN | LEU | SER | |
| 13 | GLY | LEU | LYS | ILE | HIS | SER | ASN | LEU | TYR | HIS |
Samples:
sample_1: A degron-tagged Ig2D5, [U-100% 13C; U-100% 15N], 0.3 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment
NMR spectrometers:
- Bruker AVIII-800 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts